摘要
利用甘露糖-Sepharose 4B亲和柱从半夏总蛋白中分离纯化得到一种12kD的半夏凝集素,经质谱分析明确其为半夏凝集素单体。将该12kD凝集素单体作用于体外培养的HeLa细胞,MTT法检测细胞活性并用倒置相差显微镜观察凝集素对HeLa细胞生长状态的影响。结果显示:低浓度(0.004、0.02、0.1mg/mL)半夏凝集素具有一定促进HeLa细胞增殖的作用;高浓度(0.5、1mg/mL)半夏凝集素有一定抑制Hela细胞增殖的作用。
One kind of Pinellia ternata lectin is isolated from the total protein of Pinellia ternata via mannose-Sepharose 4B affinity chromatography. The cultured Hela cell added with different concentrations of lectin is detected via the MTT method and observed with an inverted phase contrast microscope. The re- sults showed that low concentrations （0. 004 mg/mL, 0.02 mg/mL, 0. 1 mg/mL） of Pinellia ternata lec- tin can promote the proliferation of Hela cells, but high concentrations （0. 5 mg/mL, 1 mg/mL） of Pinellia ternata lectin can inhibit the proliferation of Hela cells.
出处
《浙江理工大学学报（自然科学版）》
2012年第6期858-862,共5页
Journal of Zhejiang Sci-Tech University(Natural Sciences)
基金
国家自然基金资助项目(30721712)
关键词
半夏凝集素
亲和柱
分离纯化
HELA细胞
MTT法
Pinellia ternata lectin
affinity column
isolation and purification
Hela cell
MTT method
