摘要
目的探讨脑源性神经营养因子（BDNF）对慢性不可预见性温和应激（CUMS）大鼠行为和海马胶质纤维酸性蛋白（GFAP）表达的影响。方法将30只雄性SD大鼠采用随机数字法分为正常对照＋生理盐水（对照＋S）组、CUMS＋生理盐水（CUMS＋S）组、CUMS＋BDNF组，每组各10只；采用21dCUMS制造抑郁大鼠模型，在应激第23，29，36，43天进行BDNF（1μg／kg）或生理盐水（体积分数为0．009）微量注射，并在注射前后（应激第22天和第44天）采用糖水偏好试验和旷场试验评定行为学变化，分别以蛋白印痕和荧光定量PCR法测定注射后各组大鼠海马GFAP蛋白表达和mRNA表达水平。结果（1）在BDNF注射前（应激第22天），CUMS＋S组和CUMS＋BDNF组大鼠蔗糖溶液消耗量、移动总路程、移动速度均低于对照＋S组，差异有统计学意义[F（2.28）1=5．83，P〈0．01；F（2.28）=18．45，P〈0．01；F（2．28）=20．27，P〈0．01]；在BDNF注射后（应激第44天），CUMS＋BDNF组大鼠蔗糖溶液消耗量、移动总路程、移动速度均高于CUMS＋S组，差异有统计学意义[F（2.28）=1：4．18，P〈0．01；F（2.28）=13．74，P〈0．01；F（2.28）=12．49，P〈0．01]；（2）CUMS＋S组GFAP蛋白表达（0．80±0．01）比对照＋S组（1．00±0．02）下降，差异有统计学意义[F（2.28）=2．41，P〈0．01]；CUMS＋BDNF组GFAP表达（0．88±0．04）高于CUMS＋S组，差异有统计学意义[F（2.28）=1．62，P〈0．05]；（3）CUMS＋S组GFAPmRNA水平（0．88±0．07）比对照＋S组（1．15±0．21）下降，差异有统计学意义[F（2.28）=3．68，P〈0．01]；CUMS＋BDNF组GFAPmRNA水平（1．03±0．14）高于CUMS＋S组，差异有统计学意义[F（2.28）：1．87，P〈0．05]。结论慢性不可预见性温和应激可能使正常大鼠行为表现和海马GFAP表达水平下降，而BDNF干预则可能使抑郁大鼠行为表现和海马GFAP表达水平提�
Objective To investigate the effect of brain-derived neurotrophic factor （BDNF） infusion on the rats behavioral and hippocampal glial fibrillary acidic protein （GFAP） expression after chronic unpredictable mild stress （CUMS）. Methods Thirty male SD rats were randomly divided into control ± saline （ Control ± S）, CUMS ± saline （ CUMS ± S） , CUMS ± BDNF （ CUMS ± BDNF） groups, with 10 rats in each group. Ten kinds of chronic unpredictable mild stresses were introduced for 21 days to setup the animal model of depression. BDNF （1 μg/kg） or saline （0. 009） microinjections were performed on the 23rd, 29th, 36th and 44th day during experiment. And the behavioral test （sucrose preference test and open-field test ） was carried out on the 22nd and 44th day during experiment. Hippocampal GFAP expressions were measured by Western blotting and realtime-PCR analysis. Results （ 1 ） On the 22nd day （before BDNF injection） , the behaviors in CUMS ± S group were significantly reduced as compared with Control ± S group [ F（2.28） = 5.83, P 〈 0. 01 ; F（2.28） = 18.45, P 〈 0. 01 ; F（2.28） = 20. 27, P 〈 0. 01 ] ; but on the 44th day （ after BDNF injection）, the behaviors in GUMS ± S group was significantly higher as eompared with GUMS ± S group [ F（2.28）= 4. 18, P 〈 0. 01 ; F（2.28） = 13.74, P 〈 0.01 ; F（2.28） = 12.49, P 〈0. 011. （2） GFAP gene expression in CUMS ± S group （0. 80 ± 0. 01 ） was significantly reduced as compared with Control ± S group E F（2.28） = 2.41, P 〈 0. 01 ] ; GFAP gene expression in GUMS ± BDNF group （0. 88 ±-0. 04） was signifieantly elevated as compared with CUMS ± S group [ F^2,2s） = 1.62,P 〈 0. 051. （3）GFAP mRNA level in CUMS ±S group （0. 88 ± 0. 07） was significantly redueed as compared with Control ± S group （ 1.15 ± 0. 21 ） [ F（2.28）= 3.68, P 〈 0. 01] ; GFAP mRNA level in CUMS ± BDNF group （ 1.03 -± 0. 14 ） was significantly elevated as compared with CUMS ± S gro
出处
《中华精神科杂志》
CAS
CSCD
北大核心
2012年第5期299-303,共5页
Chinese Journal of Psychiatry
基金
武汉大学认知与神经科学985工程项目（904273258）
国家自然科学基金（30971040）
关键词
脑源性神经营养因子
海马
应激
胶质纤维酸性蛋白
Brain-derived neurotrophic factor
Hippocampus
Stress
Glial fibrillary acidicprotein
