摘要
目的探讨黄芩苷(baicalin,BA)对慢性不可预知温和刺激(chronic unpredictable mild stimulus,CUMS)模型小鼠抑郁行为及细胞外调节蛋白激酶(extracellular regulated protein kinase,ERK)、环磷腺苷效应元件结合蛋白(cAMP-response element binding protein,CREB)的调节作用。方法30只癌症研究所(institute of cancer research,ICR)小鼠根据随机数字表法分为对照组(CON组)、模型组(CUMS组)、氟西汀组(FLU组)、黄芩苷高剂量组(BA-H组)、黄芩苷低剂量组(BA-L组),每组6只。除对照组外,运用CUMS方法对其余四组小鼠造模,造模共进行42 d,并从第21天开始按照分组进行灌胃至造模完成。给药结束后运用糖水偏爱实验和水迷宫实验测定小鼠的抑郁样行为,运用蛋白质印迹法(Western blot,WB)和反转录聚合酶链式反应(reverse transcription-polymerase chain reaction,RT-PCR)法分别检测小鼠海马组织中ERK、CREB mRNA和蛋白的表达情况。运用SPSS 21.0软件包,经正态检验和方差齐性检验后,多组间比较采用单因素方差分析(one-way ANOVA),两两比较采用Tukey法。结果糖水偏爱实验显示,与CON组相比,CUMS组的糖水偏爱率降低[(82.88±2.00)%,(64.49±1.24)%;t=19.11,P<0.05]。与CUMS组相比,FLU组[(81.90±1.19)%]、BA-H组[(77.86±2.51)%]、BA-L组[(67.98±2.56)%]小鼠的糖水偏爱率均增加(t=24.83,11.68,3.00;均P<0.05)。水迷宫实验结果显示,与CON组比较,CUMS组小鼠的穿越平台次数[(6.33±0.82)次,(1.83±0.75)次;t=9.93,P<0.05]和目标象限停留时间[(46.83±4.78)s,(24.25±6.12)s;t=7.13,P<0.05]均降低,逃避潜伏期延长[(14.88±3.00)s,(70.70±4.77)s;t=24.26,P<0.05]。与CUMS组相比,FLU组、BA-H组、BA-L组小鼠的穿越平台次数均增加[(5.00±0.89)次,(5.17±0.75)次,(3.33±0.82)次;t=6.64,7.67,3.31;均P<0.05],目标象限停留时间均增加[(36.80±2.66)s,(36.82±5.62)s,(33.28±3.56)s;t=4.61,3.71,3.13,均P<0.05],逃避潜伏期时间均缩短[(23.37±4.86)s,(34.83±4.72)s,(62.15±5.30)s;t=17.02,13.10
Objective To explore the regulation effects of baicalin on the behavior as well as extracellular regulated protein kinase(ERK)and cAMP-response element binding protein(CREB)in chronic unpredictable mild stimulus(CUMS)model mice.Methods Thirty ICR mice were randomly assigned to control(CON)group,model(CUMS)group,fluoxetine(FLU)group,baicalin high-dose(BA-H)group and baicalin low-dose(BA-L)group with 6 mice in each group.In addition to the CON group,the mice in the other four groups were modeled by CUMS method.The modeling was carried out for 42 days,and intragastric administration was carried out according to groups from the 21st day to the completion of modeling.After administration,the depression like behavior of mice was measured by sugar water preference test and water maze test.Western blot(WB)and reverse transcription polymerase chain reaction(RT-PCR)were used to detect the protein level and mRNA level of ERK and CREB in mouse hippocampus respectively.SPSS 21.0 was used for statistical analysis.After normal test and variance homogeneity test,one-way ANOVA was used for multi group comparison,and Tukey test was used for pairwise comparison.Results Results from the sugar preference experiment showed that compared with CON group,the sugar preference rate of CUMS group was decreased((82.88±2.00)%,(64.49±1.24)%,t=19.11,P<0.05).Compared with CUMS group,sugar preference rate in FLU group((81.90±1.19)%),BA-H group((77.86±2.51)%)and BA-L group((67.98±2.56)%)increased(t=24.83,11.68,3.00,all P<0.05).The results of water maze test showed that compared with CON group,the number of crossing platform((6.33±0.82),(1.83±0.75),t=9.93,P<0.05)and the target quadrant residence time((46.83±4.78)s,(24.25±6.12)s,t=7.13,P<0.05)of mice in CUMS group were decreased,but the the escape latency was prolonged((14.88±3.00)s,(70.70±4.77)s,t=24.26,P<0.05).Compared with CUMS group,the number of crossing platform((5.00±0.89)times,(5.17±0.75)times and(3.33±0.82)times,t=6.64,7.67,3.31,all P<0.05),and the residence time in the target
作者
贾志霞
杨佳丽
李刚刚
曹卓青
肖志刚
芦晔
孙国强
裴林
Jia Zhixia;Yang Jiali;Li Ganggang;Cao Zhuoqing;Xiao Zhigang;Lu Ye;Sun Guoqiang;Pei Lin(Hebei University of Chinese Medicine,College of Integrated Traditional and Western Medicine,Shijiazhuang 050051,China;Hebei Provincial Key Laboratory of Turbidity,Hebei Academy of Chinese Medicine,Shijiazhuang 050011,China)
出处
《中华行为医学与脑科学杂志》
CAS
CSCD
北大核心
2021年第8期679-685,共7页
Chinese Journal of Behavioral Medicine and Brain Science
基金
国家重点研发计划项目(2017YFC1701701)
河北省中医药管理局科研项目(2020176)
河北省政府资助临床医学优秀人才计划(360601)。
