摘要
目的评价血管紧张素转换酶抑制剂贝那普利及血管紧张素受体拮抗剂缬沙坦对自发性高血压大鼠(SHR)血清性激素、性腺指数及勃起功能的影响。方法将24只11周龄的雄性SHR随机分为3组:缬沙坦组[缬沙坦30mg/(kg·d)溶于0.5mL双蒸水灌胃,A组,n=8],贝那普利组[贝那普利10mg/(kg·d)溶于0.5mL双蒸水灌胃,B组,n=8],SHR对照组(双蒸水0.5mL/d灌胃,C组,n=8)。另设雄性WKY组(双蒸水0.5mL/d灌胃,D组,n=8)。适应环境1周后,测量大鼠初始体质量、收缩压及血清睾酮、血清雌二醇水平;2月后再次测量体质量及收缩压,并通过多道生理信号采集及处理系统测量0、2、5V电刺激时的阴茎海绵体内压(ICP)及平均动脉压(MAP),评估勃起功能(ICP/MAP)。颈动脉收集血样本后处死大鼠,迅速摘取睾丸称重并计算性腺指数(性腺质量/体质量×100),并通过电化学发光法测量血清睾酮、血清雌二醇水平。结果治疗2月后,缬沙坦及贝那普利均降低SHR血压,而对血清睾酮、血清雌二醇水平无明显影响。与A、B、D组比较,C组血清睾酮[(32.39±6.23)比(43.27±9.24)、(41.71±6.81)、(39.08±8.48)nmol/L,P<0.05]降低。与B、D组比较,C组血清雌二醇[(39.14±3.89)比(35.37±1.71)、(33.04±3.98)ng/L,P<0.05]升高。在0、2、5V电刺激海绵体神经时,C组的ICP/MAP最低,D组最高。单因素直线相关分析显示,收缩压、雌二醇与睾酮比值均与ICP/MAP呈明显负相关。各组间睾丸质量与性腺指数在比较,差异均无统计学意义(P>0.05)。结论缬沙坦及贝那普利降低血压的同时不影响血清睾酮、血清雌二醇水平,对SHR勃起功能无损害。
Objective To evaluate the effects of angiotensin converting enzyme inhibitor henazapril and angiotensinreceptor antagonist valsartan on the serum sex hormones, the gonado-somatic index （GSI） and the erectile function in spontaneously hypertensive rats {SHR）. Methods Twenty-four eleven-week old SHR were distributed randomly into three groups： valsartan group [received intragastric administration of valsartan 30 mg/（kg . d} dissolved in 0.5 mL distilled water, group A, n = 8], benazapril group [received intragastric administration of benazapril 10 mg/kg . d） dissolved in 0.5 mL distiUed water, group B, n= 8] and SHR-control group {.received distilled water 0.5 mL/d, group C, n=8}. Eight WKY rats was as WKY group（received distilled water 0.5 mL/d, group D}. One week after the rats got used to the environment, their body masses, systolic blood pressure（SBP）, serum testosterone and estradiol were measured. After two months treatment, body weights and systolic blood pressure were measured again, and the intracavernous pressure （ICP） and mean arterial pressure （MAP） were measured by multi-channel physiological signal acquisition and processing system with 0, 2, 5 V of cavernous nerve electrostimu- lation. Erectile function was evaluated by ICP/MAP. All the rats were sacrificed after carotid artery blood samples were collected. The testicles were removed quickly for measuring testicular weight and GSI [（Gonad weight/ total body weight） X 100]. Serum testosterone and estradiol levels were measured by electrochemiluminescence assay. Results After two months treatment, both valsartan and benazapril reduced the blood pressure but had no effect on serum testosterone and estradiol. Compared with those of group A, B and D, the serum testosterone ofgroup C was reduced [（32.39±6.23） vs （43.27 ±9.24）, （41.71±6.81）, （39.08±8.48）nmol/L, respectively, P〈0.05]. Compared with those of group B and D, the serum estradiol of group C was increased [（39.14±3.89） vs. （35.37±1.71）,
出处
《中华高血压杂志》
CAS
CSCD
北大核心
2012年第9期858-861,共4页
Chinese Journal of Hypertension
关键词
肾素血管紧张素系统
勃起功能
高血压
自发性高血压大鼠
性腺指数
Renin-angiotensin system
Erection function
Hypertension
Spontaneously hypertensive rat
Gonado-somatic index
