摘要
为证实松材线虫Bursaphelenchus xylophilus虫株携带弱毒细菌替代强毒株系防治松树萎蔫病这一思路的可行性,从2个美国松材线虫虫株体表分离并确定了优势细菌,测定了各优势细菌的毒性和致病性,并对这些菌株进行了初步鉴定。细菌分离结果显示:MG4,MG5,MG8和MG9等4菌株为美国线虫体表的优势菌株。毒力测试表明,与中国松材线虫携带的强致病菌相比,美国松材线虫携带的4个菌株的产毒能力和致病性均较低,可以作为生防细菌的候选菌株使用。经细菌的常规染色、形态学观察及16S rDNA序列分析,MG4,MG5,MG8和MG9菌株分别被鉴定为代夫特菌Delftia tsuruhatensis,恶臭假单胞菌Pseudomonas putida,嗜麦芽窄食单胞菌Stenotrophomonas maltophilia和泛菌属1种Pantoea sp.。
To confirm that pine wood nematodes carrying attenuated bacterial strains could alleviate pine wilt disease, bacteria were isolated from two nematodes native to America. Bacterial virulence and pathogenicity were tested with pine seedlings, and preliminary identification of predominant strains was determined using bacterial staining reactions and morphology combined with 16S rDNA sequence analysis. Results of the isola- tion experiment showed that the predominant bacteria which American nematodes carried were strains MG4 （Delfiia tsuruhatensis）, MG5 （Pseudomonas putida）, MG8 （Stenotrophomonas maltophilia）, and MG9 （Pan- toea sp.） Virulence and pathogenicity results demonstrated that compared with the high virulent bacteria strains （Pseudomonas fluorescence） isolated from Chinese nematodes, toxicity and pathogenicity of bacterial strains from America were relatively low. Thus, the bacteria carried by American nematodes should be considered the candidates for biological control agents. [Ch, 2 fig. 3 tab. 20 ref. ]
出处
《浙江农林大学学报》
CAS
CSCD
北大核心
2012年第5期696-702,共7页
Journal of Zhejiang A&F University
基金
国家自然科学基金资助项目(31170600)
关键词
森林保护学
松材线虫
优势细菌
毒性
致病性
细菌鉴定
forest protection
Bursaphelenchus xylophilus
dominant bacteria
toxicity
pathogenicity
identification
