摘要
利用马尾松水培离体松枝作接种材料 ,接种消毒后的松材线虫、线虫虫体上分离到的细菌分离物GD1以及两者的混合体。结果表明 ,单独接种消毒后的松材线虫或细菌分离物GD1均不能使松枝萎蔫 ,只有两者混合接种才能使松枝发病枯死。
year-old aquacultural excised branches of Pinus massoniana were inoculated with disinfected Bursaphelenchus xylophilus,bacterium isolate GD1 isolated from the body of the nematode and the mixture of the two former,respectively.The results showed that the branches inoculated with the disinfected B.xylophilus or the bacterium isolate GD1 singly did not wilt while those inoculated with the mixture of the two former wilted and died.Therefore,it was suggested that the bacterium accompanying B.xylophilus played an important role in the pathogenesis of pine wilt disease.
出处
《植物检疫》
北大核心
2001年第6期326-328,共3页
Plant Quarantine
基金
国家林业局松材线虫治理工程项目 [财农字 (1998) 84号 ]
广东省"九五"重点项目 (982 780 79)
关键词
马尾松
水培
离体松枝
松材线虫
伴生细菌
松树萎蔫病
Pinus massoniana,aquaculture,excised branch,Bursaphelenchus xylophilus,accompanying bacterium,pine wilt disease