摘要
[ Objective] This study aimed to extract the effective ingredient of pomegranate peels and explore its whitening effect. [ Method] Pomegranate peels were used as materials for single factor experiment to determine the optimal technique for effective ingredient extraction from pomegranate peels and explore the inhibition of the extracts on tyrosinase and whitening effect of the effective ingredient of pomegranate peels. [ Result] The optimal conditions for ultrasound-assisted extraction of effective ingredient in pomegranate peels were： liquid-to-solid ratio of 1：20 with 60% ethanol, extraction temperature of 60℃, ultrasonic intensity of 7 and pro- cessing time of 40 min, which led to relatively strong inhibition effect of pomegranate peel extracts on tyrosinase. The inhibition rate of extracts obtained with com- mon solvent reflux extraction method was only around 60%, while the inhibition rate of extracts obtained with ultrasound-assisted extraction method on tyrosinase ac- tivity had achieved around 90%, showing a very significant the difference, which indicated that during the process of extraction of effective ingredient from pome- granate peels, appropriate ultrasound-assistance was advantageous to the extraction of active ingredients; in addition, the inhibition effect of extracts on tyrosinase had increased with the increasing concentration of extracts, and the inhibition rate could achieve 100%. [ Condusion] This study provided a certain theoretical ba- sis for the utilization of pomegranate peels as natural green materials to develop whitening cosmetics.
[ Objective] This study aimed to extract the effective ingredient of pomegranate peels and explore its whitening effect. [ Method] Pomegranate peels were used as materials for single factor experiment to determine the optimal technique for effective ingredient extraction from pomegranate peels and explore the inhibition of the extracts on tyrosinase and whitening effect of the effective ingredient of pomegranate peels. [ Result] The optimal conditions for ultrasound-assisted extraction of effective ingredient in pomegranate peels were： liquid-to-solid ratio of 1：20 with 60% ethanol, extraction temperature of 60℃, ultrasonic intensity of 7 and pro- cessing time of 40 min, which led to relatively strong inhibition effect of pomegranate peel extracts on tyrosinase. The inhibition rate of extracts obtained with com- mon solvent reflux extraction method was only around 60%, while the inhibition rate of extracts obtained with ultrasound-assisted extraction method on tyrosinase ac- tivity had achieved around 90%, showing a very significant the difference, which indicated that during the process of extraction of effective ingredient from pome- granate peels, appropriate ultrasound-assistance was advantageous to the extraction of active ingredients; in addition, the inhibition effect of extracts on tyrosinase had increased with the increasing concentration of extracts, and the inhibition rate could achieve 100%. [ Condusion] This study provided a certain theoretical ba- sis for the utilization of pomegranate peels as natural green materials to develop whitening cosmetics.
