摘要
目的采用免疫功能低下小鼠模型探讨灵芝孢子油免疫调节作用机制。方法将昆明小鼠随机分成模型对照组、灵芝孢子油组、正常对照组,每组10只;模型对照组、灵芝孢子油组分别腹腔注射40mg.kg-1.-1)环磷酰胺建立免疫功能低下小鼠模型,正常对照组腹腔注射生理盐水(0.9%)0.1ml/10g BW;同时灵芝孢子油组150mg/kg,正常和模型对照组灌胃玉米油0.1ml/10g BW,灌胃1次/A,连续30d;用ELISA试剂盒检测小鼠血清TNF-α、IFN-$水平,半定量PCR检测小鼠脾脏、胸腺IL-2、IL-10、IL-12、IL-4、IFN-$、TNF-α基因表达量,初步探讨灵芝孢子油的免疫调节机制。结果灵芝孢子油明显提高环磷酰胺免疫低下模型小鼠血清中TNF-α、IFN-$的含量,脾脏和胸腺中IL-2、IL-10、IL-12、IL-4、TNF-α和IFN-$mRNA的表达量,与模型对照组间差异显著(P≤0.05)。结论灵芝孢子油通过增强细胞因子的分泌、表达进行免疫调节。
Objective To study the rudimentary immunoregulatory mechanisms of Ganoderma spore oil on immunocompromized mice model. Methods Thrity KM mice were randomly selected and assigned into three groups （ ten animals per group） ：the model control group, Ganoderma Lucidum spores oil group and the normal control group. The model control group and Ganoderma Lucidum spores oil group were injected intraperitoneally with cyclophosphamide at 40mg· kg-1 ·d -1 to generate a immunocompromized mice model. The normal control group were administered with 0.9% NaC1 solution 0. lml/10g BW as placebo. All agents were given orally once a day, given for consecutive 30 days, Ganoderma Lucidum spores oil group 150mg/kg, the others given maize 0. 1 ml/10g BW. The serum TNF-α, IFN-γontent of the mice through ELISA kit and the expression levels of IL-2, IL-10, IL-12, IL-4, IFN-γ, TNF-α mRNA in mouse spleen and thymus were examined
出处
《卫生研究》
CAS
CSCD
北大核心
2012年第5期833-839,共7页
Journal of Hygiene Research
基金
2009年湖南省博士后基金资助项目
关键词
灵芝孢子油
环磷酰胺
免疫调节
基因表达
作用机制
Ganoderma lucidum spore oil,cyclophosphamide,immunoregulation,gene expression,mechanisms(orally given maize)
