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雷公藤角斑病菌产毒条件研究 被引量:4

Effects of culture condition on production of toxin from Pseudocercospora elaeodendri
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摘要 对雷公藤角斑病菌福木假尾孢(Pseudocercospora elaeodendri)毒素的生物活性进行测定。结果表明,该菌所产生的毒素对雷公藤叶片具有毒性。生物活性测定方法选择离体叶片针刺法。对雷公藤角斑病菌产毒条件研究表明,该菌产毒的最佳培养液为查彼+培养液,最适pH值为7.0,适宜温度为29℃,并在全黑暗条件下充分震荡(120 r.min-1)培养25 d。 Bioassay results of the study indicated that toxin from Pseudocercospora elaeodendri had toxicity on the leaves of Tripterygiurn wilfordii Hook. f. , and the pricking and spreading method was a more effective and convenient method. The toxin-producing capability of the fungus was observed, and the results showed the cultural conditions for producting toxin were remarkable. The most optimum medium for the activity was Czapek +, and the favorable temperature was 29 ~C, the optimum pH was 7.0, cultured period being 25 days, rotating speed 120 r.min-', darkness and enough dissolved 02.
出处 《福建林学院学报》 CSCD 北大核心 2012年第4期350-354,共5页 Journal of Fujian College of Forestry
基金 福建省科技厅重点资助项目(2010N0001)
关键词 雷公藤 角斑病 福木假尾孢 毒素 产毒条件 Tripterygium wilfordii Hook. f. angular leaf spot Pseudocercospora elaeodendri toxin toxin-producing conditions
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