摘要
对从不同地点采集到的11个样品进行分离筛选,从中筛选出27株以胆固醇为唯一碳源的菌株,确定其中1株胞外胆固醇氧化酶高产菌株,其胆固醇氧化酶的酶活力为839.35 U/L。综合形态学和生理生化实验及16SrDNA序列分析结果,确定该菌株属于施氏假单胞菌(Pseudomonas.stutzeri)。
Twenty-seven strains using cholesterol as the sole carbon source were isolated from eleven samples collected from different locations. One strain with high yield of extracellular cholesterol oxidase was screened. The highest activity of cholesterol oxidase was 839.35 U/L. The strain was identified as Pseudomonas.stutzeri through analyzing its morphology, physiological and biochemical characteristics, and Sequence analysis of 16S rDNA.
出处
《食品工业》
北大核心
2012年第10期13-16,共4页
The Food Industry
关键词
胆固醇
胆固醇氧化酶
筛选
cholesterol
cholesterol oxidase
screening
