摘要
目的研究人乳铁蛋白对X射线照射所致小鼠急性辐射损伤的影响。方法无特定病原体级Balb/c雄性小鼠51只,随机分成3组,即对照组、辐射组和乳铁蛋白组。辐射组和乳铁蛋白组小鼠给予2.0 Gy X射线全身均匀照射,对照组不予照射。照射后2 h,乳铁蛋白组每只腹腔注射人乳铁蛋白4.0 mg;对照组和辐射组小鼠腹腔注射生理氯化钠溶液各1.0 ml。观察人乳铁蛋白对腹腔巨噬细胞吞噬指数和吞噬百分率、周围血象、血清超氧化物歧化酶(SOD)活力、丙二醛(MDA)水平和脏器指数的影响。结果与辐射组比较,乳铁蛋白组小鼠腹腔巨噬细胞吞噬指数和吞噬百分率、血清SOD活力和脾脏指数均显著升高(P<0.05,P<0.01),血清MDA水平显著降低(P<0.01);周围血白细胞计数、红细胞计数、血小板和胸腺、肝脏指数比较,差异无统计学意义(P>0.05)。结论人乳铁蛋白对小鼠急性辐射损伤具有一定的保护作用。
Objective To study the effect of human lactoferrin on acute radiation injury induced by X-ray in mice. Methods 51 Balb/c male mice were randomly divided into 3 groups as a control, an irradiated and a lactoferrin group. The irradiated and lavtoferrin group were given 2.0 Gy X-rays. 2 hours later, the mice in lactoferrin group received intraperitoneal injection of 4.0 mg human lactoferrin, the mice in the control and irradiated groups received 1.0 ml saline. Phagocytic and phagocytic percentage of macrophage, SOD, MDA, peripheral hemogram and viscera index were observed. Results Compared with those of the irradiated group, the phagocytic index and phagocytic percentage of macrophage, SOD, splenic index in lacto- ferrin group increased significantly （ P 〈 0. 05 or P 〈 0.01 ）, while the level of MDA reduced significantly （ P 〈 0.01 ）, WBC, RBC, PLT, thymus and liver index remained unchanged （P 〉 0.05）. Conclusion Human lactoferrin could have protective effect on acute radiation injury in mice.
出处
《中国职业医学》
CAS
北大核心
2012年第5期384-386,共3页
China Occupational Medicine
基金
山东省科技攻关计划项目(2010GSF10251)
关键词
乳铁蛋白
辐射损伤
腹腔巨噬细胞
阿氏液
抗氧化
Lactoferrin
Radiation injury
Peritoneal macrophages
Alsever＇s solution
Anti-oxidation
