摘要
目的建立实验性高脂血症恒河猴模型,为人类高脂血症研究提供理想动物模型。方法设计高脂实验饲料配方并检测常规营养成分,用高脂饲料诱导实验性高脂血症恒河猴模型,分别在2个月、4个月、6个月后监测体重、血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)等指标,对其血脂指标的变化趋势进行分析。结果模型组在实验2个月、4个月时的日采食量和摄入能量均低于对照组(P<0.05),但体重则无明显变化(P>0.05);而在实验6个月时日采食量和摄入能量与对照组无显著性差异(P>0.05),体重有显著性差异(P<0.05);模型组与对照组的TC、TG、HDL-C、LDL-C在实验前和2个月比较无显著性差异(P>0.05);在4个月比较时TC、TG、LDL-C有显著性差异(P<0.05),而HDL无显著性差异(P>0.05);在6个月比较时TC、LDL-C有显著性差异(P<0.05),并且TC、LDL-C远远高于恒河猴血脂的正常参考值水平,而TG、HDL-C则无显著性差异(P>0.05);变化趋势分析结果为,模型组TC和LDL-C水平有递增趋势、TG水平则出现了先递减后逐渐恢复的趋势、HDL-C水平未出现明显变化趋势。结论模型组恒河猴具有高胆固醇血症和高低密度脂蛋白血症的特征,已经初步建立了实验性恒河猴高脂血症动物模型。
Objective To establish Rhesus monkey model of hyperlipidemia to provide reliable animal model for the study of human hyperlipidemia. Methods Designed high fat experimental feed formulations and detected its nutrients to feed Rhesus monkey to arouse hyperlipidemias. 2 mouths,4 mouths,6mouths later, we monitored the body weight, serum total cholesterol（ TC ）, triglyceride （ TG ）, high-density lipoprotein cholesterol （HDL-C）, low density lipoprotein cholesterol （LDL-C） ,and analyzed the trend of each index above. Results When it was 2 moths and 4 months later,the feed intake and energy intake of model group were lower than control group（P 〈 0. 05 ）, but the body weig^ht of model group was not significantly different from that of the control group（ P 〉 0.05 ） ;When it was 6 moths, the feed intake and energy intake of model group was not significantly different from that of the control group, and the body weight of model group were significantly different from that of the control group（ P 〈 0. 05 ） ; When it was 2 moths later, the indexes of TC, TG, HDL-Cand LDL-C of the model group were not significantly different from that of the control group （ P ~〉 0.05 ）. When it was 4 moths later, the indexes of TC,TG, LDL-C of the model group were significantly different from that of the control group（ P 〈 0.05 ）. When it was 6 moths later, the indexes of TC, LDL-C of the model group were significantly different from that of the control group（ P 〈 0. 05 ） , and much higher than the normal levels, but the indexes of TG, HDL-C were not significantly different from that of the control group （ P 〉 O. 05 ）. According to the monitoring, TC and LDL-C showed a increase trend, TG showed a gradual recovery after the first descending trend, and the HDL-C was not significantly changed. Conclusion Rhesus monkeys of the model group has a feature of high cholesterol and high low-density lipoprotein cholesterol. The paper has initialy established Rhesus monkey model of experimental hype
出处
《中国比较医学杂志》
CAS
2012年第9期31-35,共5页
Chinese Journal of Comparative Medicine
基金
云南省技术创新人才培养基金(2009CI117)
云南省应用基础研究面上项目资助课题(2009ZC185M)
关键词
血脂
高脂血症
模型
动物
恒河猴
Lipid
Hyperlipidemia
Model, animal
Rhesus monkey
