摘要
目的:探讨ω-3脂肪酸对SW480结肠癌干细胞增殖及血管内皮生长因子(VEGF),syndecan-1,Ki-67基因表达的影响。方法:用流式细胞仪分选出SW480细胞中的CD133+/CD44+/ESA+细胞亚群(SW480结肠癌干细胞),将结肠癌干细胞分用系列浓度(15,30,60μg/μL)的EPA(二十碳五烯酸),系列浓度(15,30,60μg/μL)的DHA(二十二碳六烯酸)及溶剂处理(对照组),用MTT法检测各组细胞的增殖情况;并用RT-PCR检测DHA(60μg/μL)或EPA(60μg/μL)处理后结肠癌干细胞VEGF,syndecan-1和Ki-67表达的变化。结果:低浓度(15μg/μL)的DHA和EPA对结肠癌干细胞的生长无明显影响(均P>0.05),中、高(30,60μg/μL)浓度的EPA和DHA能明显抑制结肠癌干细胞的生长,与对照组比较差异均有统计学意义(均P<0.05)。60μg/μL的EPA及DHA能明显抑制syndecan-1和Ki-67基因的表达,与对照组比较差异均有统计学意义(均P<0.05),但两者对VEGF基因的表达无明显影响(均P>0.05)。结论:ω-3脂肪酸可抑制SW480结肠癌干细胞的增殖,其机制可能与抑制syndecan-1和Ki-67的表达有关。
Objective： To investigate the effect of ω-3 fatty acids on the proliferation and gene expressions of vascular endothelial growth factor （VEGF）, syndecan-1 and Ki-67 of SW480 colon cancer stem cells. Methods： The CD133＋/CD44＋/ESA＋ cell subgroups （SW480 colon cancer stem cells） in the SW480 colon cancer cells were sorted by flow cytometr）n Then, the colon cancer cells were treated with serial concentrations （15, 30, 60 μg/gL） ofEPA （eicosapentaenoic acid） or serial concentrations （15, 30, 60 μg/μL） ofDHA （docose hexaenoie acid）, or vehicle （control）, and the cell proliferations of each cell group were determined by MTT assa）n Furthermore, the gene expressions ofVEGF, syndecan-1 and Ki-67 in colon cancer cells after treatment of EPA （60 μg/μL） or DHA （60 μg/μL） were analyzed by RT-PCR method. Results： Both low concentrations of EPA and DHA had no noticeable effect on the growth of colon cancer cells （both P〈0.05）, while either medium or high concentration （30 and 60 μg/μL） of EPA and DHA significantly inhibited the growth of colon cancer cells, which had statistical differences compared with control group （all P〈0.05）. Both EPA and DHA at a concentration of 60μg/μL）could significantly suppress the gene expression of syndecan-1 and Ki-67 （both P〈0.05）, but showed no evident effect on VEGF gene expression （both P〉0.05）. Condusion：ω-3 fatty acids can inhibit the proliferation of SW480 colon cancer stem cells, and the mechanism is probably associated with its suppression ofsyndecan-1 and Ki-67 expression.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2012年第10期1227-1230,共4页
China Journal of General Surgery
