摘要
目的:检测PrEN、VEGF在非小细胞肺癌(N$CLC)中的表达,以MVD判断肿瘤血管生成,分析PTEN、VEGF与NSSCLC的关系及临床意义。方法:应用免疫组化SABC法检测74例NscLC组织及癌旁正常组织中PTEN、VEGF、MVD的表达。结果:PTEN在NSCLC及癌旁组织中阳性表达率为45;9%、89.2%(P〈0.05),VEGF阳性率为67.6%、20.3%(P〈0.05);FIEN阳性表达与肿瘤分化程度、临床分期及淋巴结转移相关(P〈0.05)。VEGF阳性表达与临床分期及淋巴结转移相关(P〈0.05);VEGF阳性、PTEN阴性癌组织MVD值高于VEGF阴性、PTEN阳性癌组织(P〈0.05)。PTEN与VEGF表达呈负相关(r=-0.574.P〈0.05)。结论:NSCLC中PTEN低表达、VEGF高表达与肿瘤分化程度、淋巴结转移等生物学特性有关.可能参与NSCLC漫润、转移,PTEN、VEGF及MVD联合检测可以为NSCLC早期诊断提供标志物。
Objective: The study is to judge tumor angiogenesis by MVD,and to analyze the correlation of the two genes with NSCLC and their clinical significance by detecting the expression of tumor - suppressor genes PTEN and VEGF in non -small cell lung cancer (NSCLC) organization. Methods: The expression of microvazeular density (MVD) and PTEN,VEGF were detected in 74 cases of NSCLC tissues and adjacent tissues by SABC imununohistechemical method. Results: The positive expression rates of PTEN in NSCLC tissues and adjacent tissues were 45.9% (34/74) ;89.2% (66/74) ( P 〈 0.05 ) ,and the positive expression rates of VEGF were 67.6% (50/ 74 ) .20.3 % ( 15/74 ) ( P 〈 0.05 ) ; The positive expression of PTEN was correlated with degree of differentiation, clinical stage and lymphatic metastasis ( P 〈 0.05 ), and the positive expression of VEGF was correlated with clinical stage and lymphatic metastasis ( P 〈 0.05 ) ; The expression of PTEN was negatively correlated with the expression of VEGF at the protein level( r = - 0. 574, P 〈 0.05 ). In NSCLC, the value of MVD of VEGF - negative, PTEN - positive group was lower than that of VEGF - positive, PTEN -negative group( P 〈 0.05 ). Conclusion: The higher expression of VEGF and the lower expression of PTEN were correlated with malignant biological behavior of NSCLC ,which contribute to invasion and metastasis. The detection of PTEN ,VEGF and MVD may find markers for early diagnosis of NSCLC.
出处
《成都医学院学报》
CAS
2012年第02Z期12-12,14,共2页
Journal of Chengdu Medical College
关键词
非小细胞肺癌
抑癌基因
PTEN
血管内皮细胞生长因子
微血管密度
Nonsmall -cell lung cancer
Tumor-suppressor genes
PTEN
Vascular endothelial cell growth factor
Microvascular density