摘要
目的考察氯胺酮对大鼠肝微粒体内细胞色素P450_2B酶活性的影响。方法以10mg.kg-1.d-1氯胺酮灌胃给予大鼠,连续给药10d。在第11d处死大鼠,以钙离子沉淀法制备肝微粒体。采用Omura和Sato方法测定细胞色素P450含量,采用7-戊氧基试卤灵-O-去烷基反应测定细胞色素P450_2B酶的活性。结果给药组和对照组细胞色素P450的总含量分别是(0.679±0.216)nmol·mg-1和(0.398±0.109)nmol·mg-1,细胞色素P450_2B酶的活性分别是(13.40±3.15)pmol·min-1.mg-1和(7.04±2.09)pmol·min-1.mg-1。结论氯胺酮能够增加大鼠肝微粒中细胞色素P450含量,并能显著诱导细胞色素P450_2B酶的活性。
Objective To investigate the effect of ketamine on cytochrome P450_2B activity in rat liver microsomes. Methods The rats were intragastric administrated with 10 mg·kg^-1·d^-1 ketamine for 10 days. The liver microsomes was prepared by precipitated with calcium ion after the rats were executed on the 11th day. The contents of cytochrome P450 was measured according to the method of Omura and Sato. The O-dealkylation activity of pentoxyresorufin was monitored by fluorescence spectrophotometry, which was employed as the activity of cytochrome P450_2B. Results For ketamine-treated group and saline-control group, the contents of cytochrome P450 were（0. 679±0. 216）nmol · mg^-1 and（0. 398±0. 109）nmol · mg^-1 ,and the activities of cytochrome P450_2B were（13.40±3. 15） pmol·min 1· mg^-1 and（7.04± 2.09） pmol ·min-1 ·mg^-1. Conclusions The cytochrome P450 content in rats＇ liver microsomes were increased and the cytochrome P450_2B activity was induced significantly after repetitive administration with ketamine.
出处
《成都医学院学报》
CAS
2012年第3期383-385,共3页
Journal of Chengdu Medical College
基金
国家自然科学基金项目(NO:30973369)
成都医学院基金(NO:CYZ08-006)
