摘要
【目的】分析百合根系分泌物中的主要物质,为预防百合连作障碍和建立科学栽培制度提供依据。【方法】分别用石油醚、乙醚、乙酸乙酯、氯仿和甲醇分离水培法收集百合根系分泌物,用GC-MS分析经生物检测化感强势组分(甲醇组分、石油醚组分、氯仿组分)中的有机物质。【结果】按照相似度达80%、相对含量达0.20%分析检出物质,从百合根系分泌物甲醇组分中共检出11种物质,主要为苯甲酸酯类衍生物,如邻苯二甲酸二异辛酯(52.11%)和双-2-乙己基邻苯二甲酸酯(40.95%),其他成分含量均在1%以下;从石油醚组分中检出6种苯甲酸酯类衍生物,如邻苯二甲酸二异辛酯(88.04%)、邻苯二甲酸二丁酯(9.06%),其他成分含量均在1%以下;氯仿组分中有机物质丰富,检出的有酯类、醛、酚、烯烃、烷烃等19种,含量在1%以上的有邻苯二甲酸二异辛酯(39.87%)、双-2-乙己基邻苯二甲酸酯(39.62%)、十六烷(1.36%)、2-甲氧基-1-(2-硝乙烯基)-3-苯甲氧基-苯(1.22%)、2,4-二叔丁基苯酚(1.02%)。百合根系分泌物的乙醚和乙酸乙酯组分化感作用不强,未对其中的有机物质做进一步鉴定。【结论】百合根系分泌物的GC-MS检出物主要为酯类物质,不同组分中含量高的均为苯甲酸酯类衍生物,还有少量醛、酚、烯等。
【Objective】 The study was to investigate the main organic chemicals in lily root exudates to provide scientific basis for preventing of continuous cropping obstacles and establishing of lily cropping system.【Method】 Lily root exudates collected by hydroponic culture of lily bulbs was isolated with petroleum ether,diethyl ether,ethyl acetate,chloroform and methanol respectively.The strong allelopathic components selected by bioassay were identified by GC-MS.【Result】 Based on the standard of similar degree no lower than 80% and relative content no lower than 0.20%,11 organic compounds were identified in methanol component,mainly derivatives of parabens such as 1,2-benzenedicarboxylic acid,diisooctyl ester（52.11%） and bis（2-ethylhexyl） phthalate（40.95%）,and the contents of other compounds were lower than 1%.Six derivatives of parabens were identified in petroleum ether component,such as 1,2-benzenedicarboxylic acid,diisooctyl ester（88.04%）,bis（2-ethylhexyl） phthalate（9.06%）,and the contents of other parabens were lower than 1%.There were abundant organic compounds identified in chloroform component,totally 19 compounds included esters,aldehyde,phenol,olefin,alkane,and so on.Among them,the contents of the following compounds were over than 1%：1,2-benzenedicarboxylic acid,diisooctyl ester（39.87%）,Bis（2-ethylhexyl） phthalate（39.62%）,hexadecane（1.36%）,2-methoxy-1-（2-nitroethenyl）-3-（phenylmethoxy）-benzene（1.22%）,phenol,2,4-bis（1,1-dimethylethyl）（1.02%）.The allelopathy of diethyl ether component and the ethyl acetate component were weak and the organic compounds in the two components were not further identified.【Conclusion】 The organic compounds in lily root exudates identified by GC-MS are mainly esters.The higher level compounds in all the three components are the derivatives of parabens.Also there are few aldehyde,phenol and olefin.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2012年第9期202-208,共7页
Journal of Northwest A&F University(Natural Science Edition)
基金
国家自然科学基金项目(31171949)
国家"十一五"科技支撑计划项目(2006BAD07B02)
