摘要
目的探讨比较荧光定量RT-PCR、普通RT-PCR和细胞培养3种方法在流感病毒检测中的应用,从而确定每种方法各自的实用领域。方法通过荧光定量RT-PCR、普通RT-PCR和狗肾传代细胞(MDCK)培养3种方法检测398件流感样病例(ILI)咽拭子样本。比较3种方法的灵敏度和特异性。结果实时荧光RT-PCR检测阳性样本129件(阳性率为32.4%);普通RT-PCR检测出阳性样本93件(阳性率为23.4%);MDCK细胞培养法分离出流感病毒64株(阳性率为16.1%)。荧光定量RT-PCR的灵敏度达到0.01TCID50,特异性强。结论无论从敏感性、特异性还是从时间上,荧光RT-PCR对于疫情的爆发更有应用价值。细胞培养法分离流感病毒对于核实疫情的实验室诊断和病原学监测具有重大意义。
Objective To compare the sensitivity and specitivity of real-time RT-PCR and RT-PCR and cell culture for influenza virus detection in order to determine which of the three methods is best suited to different uses.Methods A total of 398 throat swab samples were performed with the methods of fluorescent real-time RT-PCR,normal RT-PCR and Dog kidney cell passage（MDCK） cell culture in order to compare the sensitivity and specificity of between them.Results Of 398 throat swab samples 129 positive samples were confirmed according to method of fluorescent real-time RT-PCR at a rate of 32.4%;93 positive samples were isolated by ordinary RT-PCR at a rate of 23.4%;64 strains of influenza virus were detected by MDCK cell culture and tested positive at a rate of 16.1%.The sensitivity of fluorescent real-time RT-PCR is high to 0.01 TCID50 and it has high specificity.Conclusion The fluorescent real-time RT-PCR have more application value in outbreak of the epidemic because of its sensitivity,specificity and speed.But,cell culturing have more value for laboratory diagnosis and pathogen monitoring.
出处
《中国实验诊断学》
2012年第9期1642-1645,共4页
Chinese Journal of Laboratory Diagnosis
