摘要
选择Trizol法、热酚法、酵母RNA提取试剂盒法3种方法提取热带假丝酵母总RNA,通过对总RNA进行质量浓度和纯度分析,比较不同方法对热带假丝酵母总RNA提取的影响。结果表明:使用酵母RNA提取试剂盒法提取热带假丝酵母总RNA是较为有效且简便的方法。
Three methods including Trizol method, hot-phenol method, and Yeast RNA kit method were used for the total RNA extraction from Candida tropicalis. Based on total RNA concentration and purity, these methods were compared for their effects on total RNA extraction from Candida tropicalis. The results show the Yeast RNA kit method is the most effective and convenient method for total RNA extraction from Candida tropicalis.
出处
《实验技术与管理》
CAS
北大核心
2012年第9期48-50,共3页
Experimental Technology and Management