摘要
目的:观察全蝎蜈蚣对胶原免疫性关节炎大鼠的病理损伤改善作用。方法:大鼠随机分成6组:正常对照组、模型对照组、全蝎蜈蚣高、中、低剂量组和Ⅱ型胶原蛋白治疗组(CⅡ组),全蝎蜈蚣高中低剂量组分别给予全蝎蜈蚣混悬液0.4g/kg、0.2g/kg、0.1g/kg灌胃、CⅡ组予Ⅱ型胶原蛋白冻干粉60μg/kg灌胃;正常对照组和模型对照组分别用等容积生理盐水灌胃,连续给药40d。采用Ⅱ胶原蛋白诱导大鼠关节炎,同时就大鼠体重、关节疼痛评分、病理损伤评分及病理形态学改变进行观察。结果:在第三周后全蝎蜈蚣高中剂量(0.4g/kg、0.2g/kg)组大鼠体重明显高于模型组(P<0.01或P<0.05),第15天以后各剂量组(0.4g/kg、0.2g/kg、0.1g/kg)即可缓解CIA大鼠的关节疼痛,其疼痛评分明显低于模型组(P<0.01或P<0.05),且治疗组病理形态学可见关节软骨破坏程度减轻,少量炎细胞浸润,滑膜细胞增生,伴有纤维结缔组织增生,其病理损伤评分也明显低于模型组(P<0.01或P<0.05)。结论:全蝎蜈蚣药对可有效抑制CIA大鼠关节疼痛,促进体重增长和病理性损伤修复。
Objective： To observe Scorpio and Scolopendra ameliorate joint injury of rats with collagen-induced arthritis （CIA） or not. Methods： sixty female Wistar rats were randomly divided into 6 groups, the normal control group, the model control group, the low-dose, the middle- dose and the high-dose Scorpio and Scolopendra group, and the type II collagen group. Rats＇s arthritis was induced by collagen injected in the base of their tail on day 0 and day 7. Body weight, arthralgia score, pathological injured score and change of pathomorphology were observed. Results： The body weights of rats in the high and middle dose scorpio and scolopendra groups were higher than in the model group （ P 〈 0.01 or P 〈 0.05 ） after 3 weeks. The arthralgia score was remarkably decreased when compared with the model groups after 15 day and pathologi- cal injured score was too in the end of experiment. While it was found that the lever of articular cartilage destroy alleviated, fewer inflammato- ry cellular infiltration, synovial cellular proliferation and desmoplasia were shown in the slice of joints. Conclusion： The couple drugs of Scorpio and Scolopendra effectively suppressed joint pain and improved body weight and pathological restoration.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2012年第4期73-76,共4页
Pharmacology and Clinics of Chinese Materia Medica
基金
国家自然科学基金项目(30860377)
江西省自然科学基金项目(2009GZY0118)
江西省卫生厅科技计划(20092052)
关键词
全蝎
蜈蚣
类风湿性关节炎
scorpio（全蝎）
scolopendra（蜈蚣）
rheumatoid arthritis
pathological restoration
