摘要
目的了解不发酵糖革兰阴性杆菌中Ⅰ类整合子的存在情况,分析整合子与不发酵糖革兰阴性杆菌耐药性的关系。方法测定194株不发酵糖革兰阴性杆菌对抗菌药物的敏感性;应用兼并引物PCR方法,扩增整合子5'保守区的整合酶基因,对阳性PCR产物用限制性内切酶HinfⅠ作限制片段长度多态性(RFLP)分析进行整合子分类。结果 44株不发酵糖革兰阴性杆菌检测出Ⅰ类整合子。Ⅰ类整合子阳性的菌株对抗菌药物的耐药率普遍比整合子阴性的菌株高。结论不发酵糖革兰阴性杆菌临床分离株的耐药性强,Ⅰ类整合子与细菌的多重耐药性相关。
Objective To analyze the relationship between integron and drug-resistant phenotype of non-fermentative gram-neg- ative bacteria according to the distribution of class Ⅰ integrons in the non-fermentative gram-negative bacteria. Methods The susceptibility of 194 strains of non-fermentative gram-negative bacteria to antibiotics was tested by using bioMerieux VITEK 2. Integrons were detected by PCR using degenerate primers targeting 5r conserved regions of integrase genes. The class of inte- gron was determined via analysis of integrase PCR products by restriction fragment length polymorphism (RFLP) following di gestion with Hinf I restriction enzyme. Results Class Ⅰ integrons were identified in 44 strains of non-fermentative bacteria. The prevalence of antibiotic resistance in the class I integron positive isolates was generally higher than in the integron negative strains. Conclusions High antibiotic resistance was found in non-fermentative gram-negative bacteria. Class Ⅰ integrons are as- sociated with multi drug resistance in non-fermentative gram-negative bacteria.
出处
《中国感染与化疗杂志》
CAS
北大核心
2012年第5期377-380,共4页
Chinese Journal of Infection and Chemotherapy