摘要
目的了解肠致病性大肠埃希菌(EPEC)多重耐药菌株中整合酶基因的携带情况,研究整合子与抗生素多重耐药的相关性。方法使用血清学的方法对EPEC进行初筛,用PCR扩增EPEC毒力基因(eae,EAF,bfpA)进行确证。对确证为EPEC的细菌DNA进行提取,使用PCR方法对整合酶基因及在整合子中插入的基因盒进行扩增。EPEC药敏试验采用K-B琼脂扩散法。结果在34株EPEC中,ESBL为14株,其中在11株ESBL阳性细菌中扩增出整合子Ⅰ整合酶片段,在20株ESBL阴性细菌中,有7株扩增出相应的片段。在这所有的34株细菌中未检出整合子Ⅱ和Ⅲ。结论Ⅰ类整合子在肠致病性大肠埃希菌多重耐药菌株中最常见,是导致细菌多重耐药的一个重要因素,合理用药。
Objective To investigate the gene of integrase in enteropathogenic Escherichia coli（EPEC） and study the relationship between integrons and multi-drug resistance in EPEC. Method The primary screening tests for EPEC was performed using serological method and definitive detection was used to detect pathogenic genes （eae, EAF and b^A） of EPEC by PCR. The DNAs of the organism was extracted which were diagnosed as EPEC, and the integrase gene and gene cassette were amplified by PCR. Antibiotic susceptibility test was performed by Kirby-Bauer disk diffusion method. Result The 34 strains were all EPEC by detection of pathgenic genes （ eae, EAF and bfpA） by PCR. There were 14 ESBL positive isolates among the 34 EPECs. Among these 14 ESBL positive strains, 11 strains were positive for integmse gene,while only 7 of the 20 ESBL negative EPECs were positive for integrase gene. The class 2 and 3 integrons were not detected. Conclusion The class 1 integrons are common in multi-drug resistant EPEC. It is important to use antibiotics properly and control the transfer of resistance genes.
出处
《中国微生态学杂志》
CAS
CSCD
2010年第11期1024-1026,共3页
Chinese Journal of Microecology
