摘要
目的研究猪链球菌2型(SS2)中国高致病株05ZYH33株对BALB/c小鼠的致病性,探讨其作为SS2动物感染模型的可行性。方法用猪链球菌活菌经腹腔注射感染小白鼠,并做阴性对照。通过LD50测定、临床症状观察、病理剖检、病原分离鉴定、菌株在各脏器内定植分布检测、细胞因子测定,观察和分析小鼠感染SS2后机体的一系列改变。结果 BALB/c小鼠对05ZYH33株易感,其LD50为4×107 CFU/ml,并可区分强毒株和无毒株毒力差异;病原分离鉴定证实各脏器感染菌株均为SS2;涂板计数表明野生株05ZYH33在小鼠各脏器定植量显著高于无毒株Δcps2B;病死鼠的特征性组织病理学变化表现为典型的弥漫性血管内凝血和微血栓形成;野生株及无毒株均可引起炎症因子IL-6及趋化因子MCP-1的释放,但无毒株△cps2B诱导细胞因子水平显著低于野生株(P<0.05)。结论 BALB/c小鼠可被SS2感染并引发炎症反应。适龄SPF BALB/c小鼠是中国SS2强致病株良好的动物感染模型。
Objective Experiments were conducted to establish a mouse model of infection with Streptococcus suis type 2（SS2）.Methods BALB/c mice were infected with 1 ml（2×108 CFU） of SS2 from Sichuan Province via intraperitoneal injection.Mice were observed daily for 7 days to check for clinical manifestations.Results Mice were susceptible to SS2 and displayed clinical symptoms and pathological changes.LD50 was 4×107 CFU/ml.There were significant differences in the distribution of the wild strain and avirulent strain △cps2B in blood and organs.Histopathology indicated that the organs and tissue had typical pathological changes.Wild strains stimulated the secretion of the cytokines MCP-1 and IL-6.Conclusion Results showed that BALB/c mice can provide a good model for studying the pathological mechanism of infection with Streptococcus suis serotype 2.
出处
《中国病原生物学杂志》
CSCD
北大核心
2012年第8期561-564,614,共5页
Journal of Pathogen Biology
基金
国家自然科学基金项目(No.31170124
No.30972638
No.81071317
No.81171527
No.81172794)
江苏省自然科学基金项目(No.BK2011097
No.BK2010025
No.BK2010114
No.BK2010113)
