摘要
目的研究并优化类风关巴布剂中雷公藤甲素的提取方法,并测定其含量。方法 HPLC测定类风关巴布剂中的雷公藤甲素含量,采用正交试验设计法考察甲醇浓度、甲醇体积、浸渍时间、提取时间对雷公藤甲素提取率的影响。结果最佳提取条件为用100 mL的60%甲醇,浸渍4 h,加热回流2 h提取效率最高。结论本方法为类风关巴布膏的质量控制提供了可靠的方法。
OBJECTIVE To study and optimize the extraction method of triptolide in rhemeutoid-arthrosis cataplasm.METHODS With the quantitative determination of triptolide in rhemeutoid-arthrosis cataplasm by HPLC method,orthogonal test was used to evaluate the extraction,factors including the methanol concentration,methanol volume,soaking time,extraction time.RESULTS The optimal condition was using 100 mL of 60% methanol,with soaking time of 4 h and refluxing time of 2 h.CONCLUSION The optimized method is reliable for the quality control of cataplasm.
出处
《中国现代应用药学》
CAS
CSCD
2012年第9期811-813,共3页
Chinese Journal of Modern Applied Pharmacy
