摘要
目的:研究血管内皮生长因子(VEGF)和碱性成纤维细胞生长因子(bFGF)与压疮的关系,从分子水平为有效防治压疮提供理论依据。方法:将SD大鼠随机分成对照组和实验组,采用磁片循环压迫法建立大鼠Ⅲ度压疮模型,HE染色观察大鼠皮肤组织病理学变化,免疫组织化学方法检测标本中VEGF和bFGF蛋白表达情况,分析二者变化的一致性。结果:VEGF和bFGF蛋白在大鼠Ⅲ度压疮组织中的表达低于压疮周边组织及正常大鼠皮肤(P<0.01),且VEGF和bFGF的变化呈一致性(κ=0.58)。结论:VEGF和bFGF在大鼠Ⅲ度压疮组织表达降低,提示其可能是Ⅲ度压疮皮肤难以愈合的潜在关键因素。
To study the effects of vascular endothelial growth factor （VEGF） and basic fibroblast growth factor （bFGF） on the molecular pathogenesis of pressure ulcer. METHODS： SD rats were randomly divided into control group and experiment group. The pressure ulcer model was established by magnetic disk circulating compression method. HE staining was used to observe the pathological changes of the skin in the rats. The expression of VEGF and bF- GF in the tissues was detected by immunohistochemical method. RESULTS： The expression of VEGF and bFGF in the tis- sues of rat III - degree pressure ulcer was lower than that in the surrounding tissues and normal skin （ P 〈 0.01 ）. The changes of VEGF and bFGF were consistent （K = 0. 58）. CONCLUSION： The expression levels of VEGF and bFGF are decreased in the tissues of rat pressure ulcer, suggesting that they may be the potential key factors in the difficult healing of pressure ulcer.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2012年第8期1504-1506,共3页
Chinese Journal of Pathophysiology
基金
浙江省自然科学基金资助项目(No.Y2111061)
关键词
压疮
磁片循环压迫
血管内皮生长因子
碱性成纤维生长因子
Pressure ulcer
Magnetic disk circulating compression
Vascular endothelial growth factor
Basicfibroblast growth factor
