摘要
目的观察IL-6对1-甲基-4-苯基吡啶(MPP+)处理的SH-SY5Y细胞的生长和pERK的影响。方法对MPP+处理的SH-SY5Y细胞进行IL-6干预,观察细胞结构形态的改变以及pERK的含量变化和定位。结果 MPP+处理的SH-SY5Y细胞系细胞活力下降;细胞系pERK上升,高峰在24h,主要定位于胞浆;IL-6干预的SH-SY5Y细胞系pERK上升,高峰在6h,多定位于胞核。IL-6可以降低MPP+处理的SH-SY5Y细胞系的凋亡,使pERK分泌高峰提前;加用ERK抑制剂U0126可以下调IL-6对MPP+处理的SH-SY5Y细胞系的影响。结论IL-6可以通过调节pERK,减少MPP+诱导的细胞损伤,促进SH-SY5Y细胞的存活。
Objective To observe the effects of IL-6 on the survival and the expression of extracellular regulated protein kinases（pERK） of SH-SY5Y cells treated by 1-methyl-4-phenylpyridinium（MPP＋）.Methods The SH-SY5Y cells treated by MPP＋were intervened with IL-6 and mophological changes and the expression and location of pERK were observed.Results After treated by MPP＋,the viability of SH-SY5Y cells decreased and the expression of pERK elevated,which peaked in 24 h and mainly located in the cytoplasm.After adminstration of IL-6,the expression of pERK was increased and peaked in 6 h and mainly located in the nucleus.IL-6 reduced the apoptosis of MPP＋-treated SH-SY5Y cells and promoted the secretion of pERK,which was downregulated by EPK inhibitor U0126 in vitro.Conclusions IL-6 can regulate the exprsssion of pERK,decrease MPP＋-induced cell injury and promote SH-SY5Y cell survival.
出处
《江苏医药》
CAS
CSCD
北大核心
2012年第16期1869-1871,F0002,共4页
Jiangsu Medical Journal
基金
镇江市卫生局科技项目(WS0907)
镇江市社会发展项目(SH2010038)
