摘要
以糠醛渣为原料进行碱性过氧化氢法预处理,采用超声波辅助酶解法,比较分析不同模式下的葡萄糖转化率及水解液残存酶活。研究结果表明:酶解2 h时辅以超声波是糖转化的最佳条件,超声波辅助酶解及分段加酶使糖产率由55.01%提高到73.35%。超声波的机械作用影响着酶的吸附与脱附,有利于其与底物的充分结合。不同超声波酶解模式对糖产率的影响较大,且U-C模式效果较好,使糖产率提高46.35%。同时,酶解前超声波可以使糖产率提高33.34%。可见,低能耗,低污染的碱性过氧化氢预处理糠醛渣超声波辅助酶解为工业化生产乙醇提供了良好的发展方向。
Ultrasound-assisted enzymatic hydrolysis of furfural residues after alkaline peroxide pretreatment was studied. The glu- cose yields and remaining enzyme activity after enzymatic hydrolysis in different modes were analyzed and compared. The results showed that ultrasound-assisted enzymatic hydrolysis after 2 h of saccharification was the optimized condition and glucose yield in this procedure was enhanced from 55.01% to 73.35% in comparison with traditional procedure. The mechanical effect of ultra- sound-assisted affected enzyme adsorption and desorption and was beneficial to enzyme combination with the substrate. The diffe- rent ultrasonic assisted enzymatic hydrolysis were believed to affect the glucose yield and the U-C mode was better than other modes. The yields of enzyme addition after ultrasonic treatment were 46.35% higher than those of enzyme addition before ultra- sonic treatment. It is clear that ultrasound-assisted enzymatic hydrolysis of furfural residues after alkaline peroxide pretreatment with low energy consumption and low pollution will provide a good development direction for the industrial production of ethanol.
出处
《林产化学与工业》
EI
CAS
CSCD
北大核心
2012年第4期47-52,共6页
Chemistry and Industry of Forest Products
基金
国家自然科学基金资助项目(31070510)
北京林业大学研究生科技创新专项计划(201208)
关键词
糠醛渣
超声波
过氧化氢
纤维素酶解
furfural residues
ultrasound
hydrogen peroxide
cellulose enzymatic hydrolysis
