摘要
目的:探讨透析法制备低相对分子质量人参糖肽的工艺,并对其理化性质、纯度与相对分子质量分布进行研究。方法:以理化性质及收率为考察指标,单因素试验对透析制备低相对分子质量人参糖肽的工艺进行考察,采用HPLC及GPC软件测定纯度与分子量分布。结果:制备工艺确定为采用截流相对分子质量1 000 Da透析袋,加入6 L水透析4 h。制备的人参糖肽总糖肽质量分数92.49%,收率18.95%,确定为相对分子质量在300-3 500 Da的混合物。结论:采用透析法制备低相对分子质量人参糖肽操作简便易行,总糖肽含量较高,相对分子质量较低。
Objective： To investigate preparation process of low molecular weight glycopeptide from Panax ginseng by dialysis method, and study on its physical and chemical properties, purity and molecular weight distribution. Method： With physical and chemical properties and yield as indexes, preparation technology of low molecular weight glycopeptide from P. ginseng by dialysis was investigated by single-factor test, purity and molecular weight distribution were determined by HPLC and GPC software. Result： Preparation process was： closure molecular weight of dialysis bag 1 000 Da, added 6 L water and dialysis time 4 h. The content of total glycopeptide from P. ginseng was 92.49% , yield was 18.95% , it was a mixture with molecular weight 300-3 500 Da. Conclusion： This preparation technology of dialysis was simple, the content of total glycopeptide was high, and molecular weight was low.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第18期14-16,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81173532)
吉林省科技发展计划项目(201201086)
关键词
人参
糖肽
透析
理化性质
高效液相色谱法
Panax ginseng
glycopeptides
dialysis
physical and chemical properties
HPLC
