摘要
目的:探讨复方甘草酸苷对脂多糖(LPS)诱导大鼠肺损伤的保护作用。方法:将健康SD大鼠随机分为正常对照组、LPS组和复方甘草酸苷低、中、高剂量组,每组10只。通过腹腔注射LPS(10mg/kg)制备大鼠急性肺损伤模型,复方甘草酸苷各剂量组于制备模型6h后腹腔注射不同剂量复方甘草酸苷(4,8或16mg/kg)。各组制备模型24h后处死大鼠,观察肺组织的病理学改变,并检测血清中炎症因子(TNF-α、IL-1β及IL-10)及肺组织中凋亡相关因子(Bcl-2和Bax)的表达水平。结果:复方甘草酸苷治疗后大鼠肺组织病理损伤减轻,血清中TNF-α和IL-1β表达显著减弱(P<0.05),IL-10表达显著增强(P<0.05);肺组织中促凋亡因子Bax的表达显著减弱(P<0.05)而抗凋亡因子Bcl-2的表达显著增强(P<0.05)。结论:复方甘草酸苷对LPS诱导的大鼠急性肺损伤具有保护作用,其作用可能是通过调控炎症反应及细胞凋亡来实现的。
Objective： To investigate the protective effects of compound glycyrrhizin on rat lung injury induced by Lipopolysaccharide（LPS）.Methods： Healthy male SD rats were randomly divided into 5 groups： control group,LPS group,compound glycyrrhizin low-dose group,middle-dose group and high-dose group.Lung injury in rats were induced by intraperitoneal injection of LPS（10 mg/kg）.The treatment groups received intraperitoneal injection of compound glycyrrhizin（4,8,16 mg/kg） 6 hours after LPS injection.All rats were sacrificed 24 hours after LPS injection.Serum were collected to detect the concentration of TNF-α,IL-1β and IL-10 by ELISA.Lung tissues were collected to detect the pathological changes through histochemistry and the expression of Bax and Bcl-2 were detected by immunohistochemistry.Results： After the treatment of compound glycyrrhizin,lung injury in rats was alleviated,and the concentration of TNF-α and IL-1β in the serum decreased significantly while the concentration of IL-10 increased significantly（P〈0.05）.Compound glycyrrhizin suppressed the expression of Bax,and promoted the expression of Bcl-2 in lung tissues（P〈0.05）.Conclusion： Compound glycyrrhizin has protective effects on lung injury in rats induced by LPS,and the underlying mechanism may be associated to its regulation on inflammation and cell apoptosis.
出处
《武汉大学学报（医学版）》
CAS
北大核心
2012年第5期627-630,634,共5页
Medical Journal of Wuhan University
关键词
复方甘草酸苷
脂多糖
急性肺损伤
Compound Glycyrrhizin
Lipopolysaccharides
Acute Lung Injury
