摘要
目的探讨肥胖乳腺癌患者血清脂联素对癌组织中Bcl-2表达的影响。方法选择病理检验确诊的乳腺癌住院患者82例,其中肥胖乳腺癌患者45例,正常体质量乳腺癌患者37例,术前抽取空腹静脉血并取血清,ELISA法测定血清脂联素含量;采用免疫组织化学观察Bcl-2蛋白在乳腺癌中的表达情况;RT-PCR法检测乳腺癌组织中Bcl-2mRNA表达。结果与正常体质量乳腺癌组血清脂联素的含量(7.46±1.04)mg/L比较,肥胖乳腺癌组血清脂联素含量(2.77±0.76)mg/L明显下降(P<0.05);肥胖乳腺癌组乳腺组织中Bcl-2mRNA的相对表达量(0.56±0.12)与正常体质量组(0.36±0.08)比较,差异有统计学意义(P<0.05);肥胖乳腺癌组乳腺组织中Bcl-2蛋白表达的平均光密度(0.25±0.08)较正常体质量乳腺癌组Bcl-2的平均光密度(0.18±0.05)显著升高(P<0.05),并且血清脂连素的含量与肥胖乳腺癌组织中Bcl-2mRNA和蛋白的表达均呈负相关(r1=-0.894;r2=-0.947;P<0.05)。结论脂联素含量的降低可能通过上调Bcl-2相关的信号通路在乳腺癌发病中有一定作用。
Objective To explore serum adiponectin affected the expression of antiapoptotic gene Bcl-2 in breast tissue in pa- tients with breast cancer. Methods Breast tissue samples from 45 cases with breast cancer,37 cases of normal weight with breast cancer,extraction hollow and take venous blood serum before the operative, the content of serum adiponectin was determined by ELISA;The expression of Bcl-2 mRNA and protein of breast tissue were detected by RT-PCR and immunohistochemistry in obese breast cancer group and normal weight breast cancer group. Results Compared with normal weight breast cancer group（7.46 ±1.04） mg/L; the content of serum adiponectin（2.77 ±0. 76）mg/L was noticeably reduced（P〈0.05） ;the expression of Bcl-2 mRNA of breast tissue in obese breast cancer group increased obviously（P〈0.05）, while the expression of Bcl-2 protein of breast tissue in obese breast cancer group increased obviously yet（P〈0.05） ;The content of serum adiponectin was negtively relevant with the ex- pression of Bcl-2 mRNA and protein in breast tissue in obese breast cancer（r^1 =- 0. 894;r^2 =- 0. 947;P〈0.05）. Conclusion The content of lower serum adiponectin maybe raise relevant signal pathway of Bcl-2 to promote the development of obesity-related breast cancer.
出处
《重庆医学》
CAS
CSCD
北大核心
2012年第28期2922-2923,2926,F0002,共4页
Chongqing medicine
基金
河南省教育厅基金资助项目(2008B310005)
