摘要
目的分析长沙市区儿童脂联素基因+45位点单核苷酸多态性(SNP)基因型的分布频率,探讨其与儿童肥胖症的相关性。方法随机抽取147例肥胖儿童及118例健康儿童,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测脂联素基因SNP+45多态性位点、双抗体夹心ELISA测定血清脂联素。同时进行腰围(WC)、腰臀比(WHR)、体脂百分比(%BF)、收缩压(SBP)、舒张压(DBP)、空腹血糖(FPG)、血清甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、空腹胰岛素水平(FINS)、稳态模型胰岛素抵抗指数(HOMA-IR)的测定。结果肥胖组和正常组的SNP+45多态性发生频率分别为40.5%和25.4%,两组间基因型分布与等位基因频率差异均有统计学意义(P<0.05)。肥胖儿童中,TT基因型与TG、GG型相比,其脂联素水平高,而%BF、HOMA-IR、TC和LDL-C水平低,差异有统计学意义(P<0.05)。结论脂联素基因SNP+45变异可能与儿童单纯性肥胖相关,T→G突变可增加儿童肥胖症的发生,同时使脂联素水平下降。
Objective To study the distribution characteristics of adiponectin gene ＋45 single nucleotide polymorphisms（SNP） in Chinese children,and to determine the role of adiponectin gene ＋45 polymorphisms in the pathogenesis of childhood obesity.Methods A total of 147 Chinese obese and 118 healthy children were randomly selected and enrolled to identify adiponectin gene SNP＋45 polymorphism by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） assay.Plasma adiponectin levels were determined using ELISA.Waist circumference（WC）,waist to hip ratio（WHR）,percentage of body fat（%BF）,systolic blood pressure（SBP）,diastolic blood pressure（DBP）,fasting plasma glucose（FPG）,serum triglycerides（TG）,total cholesterol（TC）,high density lipoprotein-cholesterol（HDL-C）,low density lipoprotein-cholesterol（LDL-C）,plasma fasting insulin（FINS）,and homeostasis model assessment of insulin resistance（HOMA-IR） were measured.Results The allelic frequency of adiponectin gene SNP＋45 in children with obesity and healthy controls were 40.5% and 25.4%,respectively.There were significant differences in the distribution of genotypes and the allelic frequency between the two groups（P〈0.05）.The plasma adiponectin levels were significantly higher,in contrast,% BF,HOMA-IR,TC and LDL-C levels were significantly lower in obese children with TT genotype than those in obese children with TG or GG genotype.Conclusions The adiponectin gene SNP＋45 polymorphism may be associated with pathogenesis of obesity in children.T→G variance may be associated an increased risk of childhood obesity and result in a decreased level of adiponectin.
出处
《中国当代儿科杂志》
CAS
CSCD
北大核心
2011年第7期539-542,共4页
Chinese Journal of Contemporary Pediatrics
基金
湖南省教育科学规划课题(XJK03AJ006)
湖南省自然科学基金(09jj5018)
关键词
脂联素
单核苷酸多态性
肥胖症
儿童
Adiponectin
Single nucleotide polymorphisms
Obesity
Child
