摘要
以1/2MS为基本培养基,分别用不同浓度梯度的蔗糖、NO3-/NH4+、丙氨酸、谷氨酸和丙酮酸处理百里香(Thymus vulgaris)再生植株30d后,测定其对脂氧合酶(LOX)活性的影响。另外,培养百里香20、30和40d后分别用茉莉酸甲酯、水杨酸和壳聚糖等外源因子对百里香进行处理,在不同的处理时间下测定LOX活性,探讨外源因素对增殖百里香中LOX活性的影响。结果表明,碳氮源调控30d百里香,蔗糖浓度为4%、NO3-/NH4+比例为2∶1时最高;前体调控添加0.6mmol.L-1苯丙氨酸和0.4mmol.L-1谷氨酸诱导LOX活性达到最大值,丙酮酸对LOX酶活几乎无影响;20d百里香经1.0mmol.L-1 MeJA喷施诱导48h后LOX活性为对照的655.4%,经150mg.L-1 SA诱导12h后LOX活性为对照的503.8%,经200mg.L-1壳聚糖诱导24h后LOX的活性为对照的429.1%。30d百里香经0.5mmol.L-1 MeJA诱导48h后LOX的活性为对照的688.1%,经150mg.L-1 SA诱导12h后LOX的活性为对照的383.8%,经200mg.L-1壳聚糖诱导24h后LOX的活性为对照的360.8%。40d百里香在外源处理因素作用下对LOX活性作用不明显。可见,在不同时期用不同处理因素对百里香再生植株进行处理能显著调节LOX的活性。
Plantlets of thyme were cultured on the 1/2 MS medium that containing surcose with different concentrations, NO3-/NH4＋ , phenylalanine, glutamic acid and pyruvic acid. The LOX activity of thyme was tested after 30 d. Furthermore, the plantlets were induced for 0-120 h by MeJA, SA and chitosan at different culture for 20 d, 30 d and 40 d, and LOX activity was tested. The results showed that the highest LOX activity was obtained when the plantlets of thyme were cultured on the 1/2 MS medium with 4% sugar and 2 ： 1 NO3-/NH4＋ ratio for 30 d. The LOX activities were increased respectively when adding 0.6 mmol . L-1 precursor regulation L-phenylalanine, 0.4 mmol . L-1 glutamic acid. The LOX activities of plantlets cultured for 20 d were 655. 4%, 503. 8% and 429. 1% more than control by adding 1. 0 mmol . L-1 MeJA,150 mg. L-1 SA, and 200 mg . L -1 chitosan. The LOX activities of plantlets cultured for 30 d were increased to 688. 1% , 383. 8% and 383. 3 % when adding 0. 5 mmol . L -1 MeJA, 150 mg . L -1 SA, and 200 mg. L-1 chitosan, respectively. The LOX activities of plantlets cultured for 40 d did not change. Therefore, the LOX activity was affected by adding different inducer at different time.
出处
《草业科学》
CAS
CSCD
北大核心
2012年第9期1390-1395,共6页
Pratacultural Science
基金
国家自然科学基金(31160087)
甘肃省科技厅自然科学基金(0803RJZA038)
关键词
百里香
脂氧合酶
诱导因素
酶活性
thyme
Lipoxygenase
inducing factors
enzyme activity
