摘要
应用植物组织培养技术，通过高效液相色谱法和分光光度法.对丹参培养细胞及其水溶性有效成分迷迭香酸进行研究。结果：①丹参培养细胞中含有迷迭香酸。②2，4-D对丹参愈伤组织的诱导有促进作用。③B5培养基有利于丹参愈伤组织的生长.KH2PO4对丹参悬浮培养细胞的生长有较大的影响。④对丹参培养细胞生长最适的蔗糖浓度为3％，对迷迭香酸形成的最适的浓度为5％。
By application of the culture technique of plant tissues, the study was conducted on culture cell of Salvia miltiorrhiza and its soluable active principle-Rosmarinic Acid. The results showed that ①Rosmarinic Acid is contained in culture cell of Salvia miltiorrhiza ;② 2,4 - D possesses the promoting function to induce the wound-healing tissue of Salvia miltiorrhiza; ③B5 culture medium is helpful for growth of the wound-healing tissue of Salvia miltiorrhiza and KH2PO4 is greatly influential to growth of suspended culture cell of Salvia miltiorrhiza and ④the most suitable concentration of sucrose for growth of culture cell of Salvia miltiorrhiza is 3% and is 5% for formation of Rosmarinic Acid .
出处
《上海中医药大学学报》
CAS
1999年第4期56-59,共4页
Academic Journal of Shanghai University of Traditional Chinese Medicine
基金
上海市教委青年基金!97QB41
上海市卫生局基金!165
