摘要
目的观察火针治疗前后大鼠跟腱末端区细胞凋亡情况的变化,探讨火针治疗末端病的作用机理,为末端病的火针治疗提供理论依据。方法采用"电击跳跃法"建立大鼠跟腱末端病模型,施以火针治疗,采用TUNEL法检测细胞凋亡指数(AI)。结果(1)1次火针治疗后,治疗组大鼠末端区主要在跟腱、纤维软骨区、腱围这三个部位的AI比对照组有不同程度降低;(2)经4次火针治疗后,跟腱、纤维软骨区、腱骨关节面软骨、腱围的AI明显低于对照组;(3)4次火针治疗后在跟腱、纤维软骨区、腱骨关节面软骨、腱围的AI明显低于1次火针治疗组。结论(1)8周的"电击跳跃法"成功建立了大鼠跟腱末端病模型。(2)1次火针治疗可以减少末端区的细胞凋亡和修复已凋亡的细胞。(3)4次火针治疗不仅使末端区的凋亡细胞得到修复,而且加速了整个末端区组织和结构的恢复。
Objective Through the terminal apoptosis＇s eomparison after four times and one time treatment at fire needle, explore the fire apoptosis mechanism at the end of treatment for the enthesiopathy, provides theory basis for enthesiopathy＇s treatment by fire needle. Methods Using＂ electric shock jump＂ at rat model, establish the enthesiopathy model, then use fire needle treatment. Using TUNEL method to detect apoptosis index （AI）. Results （ 1 ） After one time＇s fire needle treatment, the Al at heel tendon, area of fibrocartilage, surround of tendon all reduced of the treatment group＇s terminal eompared with control group. （2） After four time＇s fire needle treatment, the AI at heel tendon, area of fibrocartilage, tendon articular cartilage, surround of tendon reduced obviously compared with control group. （3） After four time＇s fire needle treatment, the AI at heel tendon, area of fibrocartilage, tendon articular cartilage, surround of tendon are lower compared with group through one time＇s fire needle treatment. Conclusions （ 1 ） Eight weeks＂ electric shock jump＂ have succeed establish the enthesiopathy model. （2）One time＇s fire needle treatment can reduce the terminal apoptosis and repair the apoptosis. （3） Four time＇s fire needle treatment can not only repaired the apoptosis, but accelerate all terminal area＇s restore.
出处
《西安体育学院学报》
CSSCI
北大核心
2012年第5期582-587,共6页
Journal of Xi'an Physical Education University
基金
北京市教委科技以及社科计划资助项目(KM200810029004)
关键词
火针
末端病
细胞凋亡指数
fire needle
enthesiopathy
apoptosis index
