摘要
目的:从分子水平探讨鸡免疫系统的个体发生学。方法:利用双重荧光定量RT-PCR(dqRT-PCR)技术,对14～35日龄主要组织相容性复合体(MHC)单倍型鸡品系G2(B15单倍型)和G5系(B5单倍型)鸡群的肺组织(包括气管)、脾脏和外周血淋巴细胞(PBL)中IFN-γ、IL-18、IL-4和IL-10的mRNA含量进行定量检测。结果:随日龄增长,两鸡群脾脏中IFN-γ转录水平升高,PBL中IL-18和IL-10整体下降。G5系鸡群脾脏中IFN-γ转录水平始终高于肺组织和PBL。两鸡群脾脏中IL-18转录水平均高于肺组织和PBL,脾脏中IL-4在孵化后早期转录水平较高,而肺组织中则出现较晚。两鸡群肺组织、脾脏和PBL中IL-10均在35日龄降到最低。结论:14～35日龄无特定病原体(SPF)鸡脾脏中IFN-γ转录水平随生长发育而增多,而肺组织和PBL中未见明显随生长发育变化的规律;PBL中IL-18和IL-10转录水平随生长发育而下降;18～35日龄,肺组织、脾脏和PBL中IL-10转录水平均随日龄增长而降低;不同MHC-B单倍型鸡群细胞因子转录水平受日龄调节相似。
Objective：To investigate the ontogeny of chicken immune system.Methods：IFN-γ,IL-18,IL-4 and IL-10 mRNA was isolated from lungs,spleens and peripheral blood lymphocytes（PBL） of genetically defined lines G2（B15/B15 haplotype） and G5（B5/B5 haplotype） chickens（14 to 35 days old）,and the levels in samples were detected by duplex quantitative real-time reverse transcription polymerase chain（dqRT-PCR）assay.Results：IFN-γ in spleens was increased with growth,while IL-18 and IL-10 in PBL were in the opposite.IFN-γ in spleens of line G5 was higher than that in lungs and PBL all the time.IL-18 in spleens of both lines was higher than that in lungs and PBL.IL-4 in spleens was higher during the early posthatch period,but later in the lungs.IL-10 in the lungs,spleens and PBL of both lines were declined to the lowest at the 35 days old.Conclusion：IFN-γ in spleens of SPF chickens（14 to 35 days old） increased with growth,but no obvious discipline shown in lungs and PBL;IL-18 and IL-10 in PBL decreased with growth.IL-10 in lungs,spleens and PBL was decreased with growing from 18 to 35 days old.The transcription level of cytokines in different MHC-B haplotype chickens were regulated similarly by the age.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2012年第8期722-727,共6页
Chinese Journal of Immunology
基金
中央级公益性科研院所基本科研业务费项目(2009-08)
兽医生物技术国家重点实验室基本科研业务费项目(NKLVBP2008)
