摘要
目的测定新诊断2型糖尿病患者血清磷酸酪氨酸衔接蛋白(APPL)1水平,分析血清APPL1水平与体质量指数(BMI)、体脂分布(WHR)、收缩压(SBP)、舒张压(DBP)、空腹血糖(FPG)、空腹血清胰岛素(FINS)、糖化血红蛋白(HbA1c)、胰岛素抵抗指数(HOMA-IR)等的关系,初步探讨新诊断2型糖尿病患者血APPL1水平的变化。方法应用酶联免疫吸附法(ELISA)对临床上新诊断的2型糖尿病患者63例(病例组)和健康对照者22例(对照组),进行空腹血清APPL1水平测定。稳态模型(HOMA-MODEL)计算胰岛素抵抗指数(HOMA-IR)。结果(1)病例组血清APPL1水平(85.71±27.39)pg/ml显著高于对照组(64.52±16.28)pg/ml,差异有统计学意义(P<0.01);(2)2型糖尿病(T2DM)组BMI、WHR、SBP、FINS、LgHOMA-IR均高于对照组(P<0.01),低密度脂蛋白胆固醇(LDL-C)高于对照组(P<0.05);而T2DM组高密度脂蛋白胆固醇(HDL-C)显著低于对照组(P<0.01);(3)APPL1与空腹血糖(FPG)、空腹血胰岛素(FINS)、胰岛素抵抗指数(LgHOMA-IR)呈显著正相关关系(r=0.215、0.297、0.334,P=0.014、0.006、0.002);(4)以APPL1为应变量进行多元线性回归分析,结果胰岛素抵抗指数(β=0.329,P=0.002)进入方程。结论新诊断2型糖尿病患者血清APPL1水平增高,APPL1可能与2型糖尿病的发生发展密切相关。
Objective To investigate serum APPL1 level in patients with newly diagnosed type 2 diabetes mellitus （T2DM） and analyze its correlation with body mass index （BMI）, waist-to-hip ratio （WHR）, blood pressure, fasting plasma glucose, fasting blood insulin, HbAlc, and insulin resistance （HOMA-IR）. Method Serum APPL1 levels were determined using ELISA in 22 normal control subjects and 63 patients with newly diagnosed T2DM, and HOMA-IR of the subjects was calculated using the HOMA model. Results The diabetic patients had significantly elevated levels of serum APPL1 compared with the control subjects （85.71±27.39 vs 64.52±16.28 pg/ml, P〈0.01）, with also significantly increased BMI, WHR, SBP, FINS, LgHOMA-IR （P〈 0.01） and LDL-C （P〈0.05） but lowered HDL-C （P〈0.01）. Fasting serum APPL1 levels were positively correlated with FPG, FINS, and HOMA-IR （r=0.215, 0.297, 0.334, P=0.014, 0.006, 0.002, respectively）. In multiple linear regression analysis with APPL1 as the dependent variable, HOMA-IR （β=0.329, P=0.002） was included in the equation. Conclusion Patients with newly diagnosed T2DM have elevated serum APPL1 levels, suggesting the involvement of APPL1 in the development of T2DM.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2012年第9期1373-1376,共4页
Journal of Southern Medical University
基金
陕西省科技攻关项目(2010K01-143)
