摘要
目的:实验旨在观察褐藻多聚糖硫酸脂对体外培养人毛乳头细胞增殖的影响。方法:采用两步酶消化法分离纯化人毛乳头细胞,取第5代细胞用于实验,选择8个浓度的海藻多聚糖硫酸酯作用于体外培养的人毛乳头细胞72h,四甲基偶氮唑蓝(MTT)法测定细胞增殖活性。结果:显示0.1～1.25mg/ml浓度范围的海藻多聚糖硫酸酯有明显的促进细胞增殖作用(P<0.05)。结论:海藻多聚糖硫酸酯在一定浓度范围内可促进毛乳头细胞增殖。
Objective To investigate the effect of fucoidan on human dermal papilla cell（DPC） in vitro.Methods DPCs were isolated and cultured by the two-step enzymic methods.The fifth generations of DPCs were selected to be incubated with eight different concentrations of fucoidan for 72 h,and then the cell proliferation was detected by using MTT assay.Results Fucoidan at the concentrations of 0.1～1.25mg/ml could significantly promote the DPC proliferation（P﹤0.05）.Conclusion Fucoidan at adequate concentrations can stimulate the proliferation of human DPCs.
出处
《中国美容医学》
CAS
2012年第9期1542-1543,共2页
Chinese Journal of Aesthetic Medicine
基金
扬州市科技攻关项目编号:(yzk2008041)
