摘要
目的:对长春花生物碱中的文多灵和长春质碱的分离工艺进行研究。方法:以薄层色谱法(TLC)为检测手段,对分离文多灵和长春质碱的展开系统、大孔吸附树脂型号、洗脱液浓度、上样液浓度、吸附洗脱性能参数进行了考察,重量法对产物文多灵和长春质碱进行定量。结果:展开系统为氯仿-乙酸乙酯-甲醇(5:3:1)最好;NKA-9型大孔吸附树脂分离效果最佳;分别用30%和55%乙醇溶液富集得到长春质碱和文多灵;上样液浓度为200 mg·ml^(-1)时,总碱的回收率最高。结论:采用NKA-9型大孔吸附树脂一步柱层析,分离长春花生物碱废渣中长春质碱和文多灵,相对于原生物碱样品重量富集率分别为11.2%和14.5%,分离快速、简便。
Objective： To study the separation process of vindoline and catharanthine in the alkaloids from Catharanthu roseus. Method ： Vindoline and catilaranthine were detected by TLC, and the specifications of the macroporous adsorption resin, eluting condi- tions, concentration of the samples, adsorption and elution parameters were investigated. Result： Chloroform-ethyl acetate-methanol（5 ：3：1 ） was the optimal solvent in the identification of vindoline and catharanthine, and macroporous adsorption resin NKA-9 showed promising profile under the conditions as follows： 30% ethanol for the elution of catharanthine, and 55% ethanol for that of vindoline. The total recovery of the alkaloids reached the highest level when the concentration of the samples was 200 mg ~ ml-l. Conclusion： The method of one-step column chromatography with macroporous adsorption resin NKA-9 is simple and rapid in the enrichment of catharanthine and vindoline with the yield of 11.2% and 14.5%. respectivelv.
出处
《中国药师》
CAS
2012年第7期923-926,共4页
China Pharmacist
关键词
文多灵
长春质碱
大孔吸附树脂
Vindoline
Catharanthine
Macroporous adsorption resin
