摘要
目的研究纤维素酶在提取长春花生物碱中的应用。方法采用酸水浸提法和酶浸提法提取长春花中的长春碱、长春质碱、文多林,高效液相色谱(HPLC)法测定其含量。结果酸水浸提法和酶浸提法提取的长春碱、长春质碱、文多林含量分别为0.0158和0.0052 mg/ml;0.0456和0.0204 mg/ml;0.0898和0.0421 mg/ml。结论在单位时间内酶提法可显著提高长春碱、长春质碱、文多林的收率,可考虑将酶解法用于工业上长春花生物碱的提取中。
Objective To study the effect of cellulose enzyme on extraction of alkaloid from Catharanthus roseus.Methods HPLC method was used to determine the contents of vinblastine,catharanthine and vindoline from C.roseus.Results Compared with the traditional acidic water extraction,the enzymatic method extraction yield of vinblastine,catharanthine and vindoline was increased respectively.Conclusion The yield of vinblastine,catharanthine and vindoline from C.roseus can be effectively increased by using enzymatic extraction process.It is available to use enzyme in extraction alkaloid from C.roseus.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2010年第3期544-545,共2页
Lishizhen Medicine and Materia Medica Research
基金
"中央级公益性科研院所基本科研业务费专项资金"
"农业部热带作物种质资源利用重点开发实验室开发基金"资助(No.PZS010)
关键词
长春花
长春碱
长春质碱
文多灵
纤维素酶
Catharanthus roseus
Vinblastine
Catharanthine
Vindoline
Cellulose enzyme
