摘要
目的评价芬太尼和瑞芬太尼对人肺癌A549细胞活力的影响。方法人肺癌A549细胞培养至对数生长期，接种于96孔培养板或75ml培养瓶中，采用随机数字表法，将其随机分为9组（n=30）：不同浓度芬太尼组（F1-4组）芬太尼终浓度分别为0．5、5．0、50．0、500．0ng／ml，不同浓度瑞芬太尼组（RF1-4组）瑞芬太尼终浓度分别为0．5、50、50．0、500．0ng／ml，正常对照组（c组j加入等容量的RPMll640培养基。分别于孵育24、48和72h时，采用噻唑蓝比色法测定细胞活力；于孵育24h时经AnnexinV—FITC／PI双标记染色后，采用流式细胞仪检测细胞凋亡率，经PI染色后，采用流式细胞仪检测细胞周期分布。结果C组、F2-4组或RF2-4组孵育72h时人肺癌A549细胞活力依次降低，孵育24h时s期比例依次降低，G2／M期比例和凋亡率依次升高（P〈0．05）。结论芬太尼和瑞芬太尼终浓度≥5ng／ml时，可浓度依赖性地抑制人肺癌A549细胞活力，其机制可能与诱导细胞凋亡，使细胞周期停滞于G2／M期有关。
Objective To investigate the effects of fentanyl and remifentanil on the viability of human adenocarcinoma cell line A549. Methods Human adenocarcinoma A549 ceils cultured in logarithmic growth phase were seeded in 75 ml culture bottles or 96-well plates. After being cultured for 24 h, the cells were randomly divided into 9 groups （n = 30 each）： 4 fentanyl groups （groups F2-4 ）, 4 remifentanil groups （groups RF2-4 ） and control group （group C）. Groups F1-4 were exposed to fentanyl with the final concentrations of 0.5, 5.0, 50.0 and 500.0 ng/ml respectively. Groups RF1-4 were exposed to remifentanil with the final concentrations of 0.5, 5.0, 50.0 and 500.0 ng/ml respectively. The viability of the ceils was determined by methyl thiazolyl tetrazolium assay after being incubated for 24, 48 and 72 h. The cell cycle progression and apoptosis were determined by flow cytometry after being incubated for 24 h. Results Compared with group C, the viability of A549 cells were gradually decreased at 72 h of incubation, the proportion of the cells in S phase was gradually decreased at 24 h of incubation, and the proportion of the cells in G2/M phase and apoptotic rate were gradually increased in groups F2-4 and in groups RF2-4 （ P 〈 0.05）. Conclusion Fentanyl and remifentanil with the final concentration ≥ 5 ng/ml can inhibit the viability of human adenoearcinoma cell line A549 in a dose-independent manner by inducing cell apoptosis and cell cycle arrest in G2/M phase.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2012年第7期817-819,共3页
Chinese Journal of Anesthesiology
基金
河北省高等学校强势特色学科（2005-52）
河北省卫生厅重点学科跟踪项目（GL200737）
河北省自然科学基金（c2008000967）
关键词
芬太尼
哌啶类
细胞系
肿瘤
细胞存活
Fentanyl
Piperidines
Cell line, tumor
Cell survival
