摘要
利用SRAP和ISSR分子标记，研究了14份耐盐茄子种质资源的遗传多样性，结果表明，2种标记均能揭示材料间较高的遗传多样性，其中ISSR标记多态性略高于SRAP标记。在SRAP分析中，每对引物组合可扩增出8-15条DNA片段，平均为12．12条：26对SRAP引物组合共扩增出315条DNA片段，其中263条具有多态性，多态性比率为83．49％；材料间遗传相似系数变化范围为0．212～0．923，平均值为0．755。在ISSR分析中，每个引物可获得5～16条DNA片段，平均为10．87条；15个ISSR引物共扩增出163条DNA片段，其中141条具有多态性，多态性比率为86．50％；材料间遗传相似系数变幅为0．333-0．957，平均值为0．736。聚类分析表明，2种标记都能将供试材料完全区分开来，聚类结果具有一定的相似性，但也存在明显差异。Mantel相关分析表明，SRAP分析与ISSR分析的相关性达到极显著性水平（r=0．904，P〈0．01）。
Genetic diversity in 14 salt-tolerant eggplant cultivars were assessed using sequence related ampli- fied polymorphism （SRAP） and inter simple sequence repeat （ISSR） makers. These results suggested that there was a high level of genetic diversity among 14 salt-tolerant eggplant cultivars, and ISSR makers were superior to SRAE In SRAP analysis, each primer could amplify 8 to 15 polymorphic bands with an average of 12.12 bands. A total of 315 bands were observed in 26 primers, among which 263 bands were polymorphic and the percentage ofpolymorphic bands （PPB） was 83.49%. Among the eggplant accessions, the SRAP-based genetic similarity （SRAP-GS） ranged from 0.212 to 0.923 with a mean of 0.755. In ISSR analysis, the number of bands from each ISSR marker ranged from 5 to 16 with an average of 10.87. A total of 163 bands were detected, among which 141 bands were polymorphic and the PPB was 86.50%. The ISSR-derived genetic similarity （IS- SR-GS） ranged from 0.333 to 0.957 with a mean of 0.736. The cluster analysis indicated that all satl-tolerant eggplant accessions could be distinguished by both SRAP and ISSR markers. Mantel test indicated that SRAP and ISSR markers were significantly associated （r=0,904, P〈0.01）.
出处
《植物生理学报》
CAS
CSCD
北大核心
2012年第8期789-794,共6页
Plant Physiology Journal
基金
上海市科技兴农重点公关项目[沪农科攻字(2009)第2-1号]
上海市自然科学基金(ZR1426800)
现代农业产业技术体系建设专向资金(zhsyz)
