摘要
目的:探讨力竭运动后不同时相心脏窦房结、房室结和浦肯野氏纤维细胞炎性因子金属蛋白酶-1基因和蛋白水平的表达特点,为运动性心律失常发生机制的阐明提供实验依据。方法:100只健康成年雄性SD大鼠随机分为一次力竭运动、反复力竭组及相应的其对照组,每组10只。分别于力竭运动后0 h、4 h、12 h及24 h取材,进行心电图、免疫荧光组化及实时荧光定量PCR分析。应用激光显微切割技术定位并收集房室结和浦肯野氏纤维细胞研究细胞炎性因子ADAMTS-1的mRNA和蛋白表达的变化。结果:一次力竭运动后房室结和浦肯野氏纤维炎性反应相关因子ADAMTS-1mRNA和蛋白即刻显著高于对照组(P<0.05)。运动后即刻窦房结、房室结、浦肯野氏纤维ADAMTS-1基因与蛋白表达明显上升,之后开始下降,4 h接近低谷,一直持续到24 h(P<0.05),以浦肯野氏纤维的蛋白改变更为显著(P<0.01)。结论:一次力竭运动后心脏传导系统各部位炎性因子ADAMTS-1在mRNA和蛋白水平大量表达,引起传导系统炎性细胞浸润,细胞间质增殖乃至纤维化,是运动性心律失常的诱发因素之一。一次力竭运动后心脏传导系统ADAMTS-1蛋白的表达以浦肯野氏纤维的改变更为明显,易影响正常心律的室内传导,构成运动性室性心律失常的发生基础。
Objective To explore the expression characteristics of sinoatrial node,atrioventricular node,Purkinye＇s fibre cell inflammatory factor MMP-1 gene and protein levels after exhaustive exercise so as to provide experimental reference to the occurrence mechanism of athletic arrhythmia.Method 100 healthy adult male SD rats were divided randomly into groups of one-time exhaustive exercise groups,repeated exhaustive groups and relative control groups.Each group has 10 rats.Samples were collected immediately,4 hours,12 hours and 24 hours after the exhaustive exercise.Electrocardiogram,immunofluorescence and real-time fluorescent quantitative PCR analysis were adopted.Using fibre laser cutting technology to position and collect the changes of mRNA and protein expression of atrioventricular node and Purkinye＇s fibre cell inflammatory factor ADAMTS-1.Result The immediate samples of mRNA and protein of atrioventricular node and Purkinye＇s fibre inflammatory factor ADAMTS-1 after one-time exhaustive exercise are significantly higher than those of the control group（P〈0.05）.Sinoatrial node,atrioventricular node and Purkinye＇s fibre ADAMTS-1 gene and protein expression rose distinctly immediately after the exercise,and then starts decreasing.After 4 hours,the indicators drop nearly to the bottom and continue until 24 hours（P〈0.05）.The changes of Purkinye＇s fibre protein are quite obvious（P〈0.01）.Conclusion Large quantity of mRNA and protein expression of the different parts inflammatory factor ADAMTS-1 of heart conduction system after one-time exhaustive exercise causes infiltration of the inflammatory cell of the conduction system,proliferation of intercellular substances and fibrosis.This is one of the risk factors for athletic arrhythmia.After one-time exhaustive exercise,clear changes of Purkinye＇s fibre protein can be seen in the protein expression of heart conduction system ADAMTS-1.This may affect intraventricular conduction of normal heart rhythm and form a base for athletic arrhythmia.
出处
《体育科研》
2012年第4期17-20,共4页
Sport Science Research
基金
国家体育总局体育科学研究所基本科研业务经费(10-01)
