摘要
目的 探讨低氧诱导因子-1α（HIF-1α）、血栓烷素B2（TXB2）、6-酮-前列腺素F1α（6-Keto-PGF1α）及透明质酸（HA）联合检测在慢性乙型肝炎（CHB）肝脏微循环障碍诊断和监测中的意义。方法 对81例CHB患者和5例健康志愿者留取血清作HA化学发光法检测,抗凝血浆作TXB2和6-Keto-PGF1α放射免疫法检测,同时肝穿取标本进行HIF-1α免疫组化染色,并用透射电镜观察肝组织超微结构的变化。结果 随着CHB分级的加重,HIF-1α在肝组织中的表达强度和范围逐渐加大,HA、TXB2逐渐上升（均P＜0.05）,6-Keto-PGF1α逐渐下降。电镜示肝窦腔内红细胞聚集,肝窦阻塞和狭窄明显,狄氏腔中胶原纤维沉积及基底膜形成率逐步加重。结论 HIF-1α、HA、TXB2及6-Keto-PGF1α联合检测能较好地反映肝脏微循环状况。
Objective To study the diagnostic method of microcirculation disturbance of patients with chronic hepatitis B（CHB）.Methods 81 patients with CHB and 5 normal volunteer were observed.Hypoxia-induciable factor was detected by immunohistochemistry stain,TXB2 and 6-Keto-PGF1α were detected by chemiluminescence and HA was detected by RIA,ultrastructure of liver was observed by transmission electron microscope（TEM）.Results Accompany with the pathological changes of liver in patients with CHB,the strength and range of expression of HIF-1α enhanced,HA and TXB2 of serum rised gradually（all P＜0.05）,but 6-Keto-PGF1α of blood plasma fell-off.The electron microscope showed that erythrocyte aggregation,stenosis and blockage of sinus hepaticus turned obviously,collagen fibers deposition and basal membrane formation aggravated gradually.Conclusion Associated detection with HIF-1α、HA、TXB2 and 6-Keto-PGF1α can reflect the condition of microcirculation of liver perfectly.
出处
《中国基层医药》
CAS
2012年第16期2415-2416,I0002,共3页
Chinese Journal of Primary Medicine and Pharmacy
基金
南京军区医学科技创新经费资助项目
关键词
肝炎
乙型
慢性
微循环障碍
低氧诱导因子
肝窦内皮细胞
Hepatitis B,chronic
Hypoxia-induciable factor
Microcirculatory disturbance
Sinusoidal endothelial cell
