摘要
目的用聚合酶链反应(PCR)检测浙江省淳安县鼠中感染巴贝西原虫的情况。方法利用PCR方法检测淳安县106份鼠肝、脾标本,对检测到的阳性片段,进行序列测定和分析。结果 PCR检测结果显示,3份标本呈阳性,其中2份来自黄胸鼠,1份来自褐家鼠;PCR产物序列与巴贝西原虫高度相似,相似率均为99%。结论浙江省淳安县鼠类中检测到巴贝西原虫DNA片段,提示该地区可能有巴贝西原虫感染。
Objective To detect the infection of Babesia protozoa in rodents in Chun’an county, Zhejiang province. Methods The polymerase chain reaction （PCR） was employed to detect the liver and spleen samples of 106 rodents from Chun’an county and the positive ones were sequenced, followed by phylogenetic analysis. Results It was found that there were three positive samples, 2 of which were obtained from Rattus tanezumi and one of which from R. norvegcus. The sequences of the positive samples were of high similarity with those from Babesia, with the rates of similarity being both 99%. Conclusion DNA segments of Babesia can be detected from rodents in some mountain areas of Zhejiang province, indicating that it is likely that there exists infection of rodents with Babesia in the area.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
2012年第4期303-305,共3页
Chinese Journal of Vector Biology and Control
关键词
鼠
巴贝西原虫
聚合酶链反应
Rodents； Babesia protozoa； Polymerase chain reaction
