摘要
目的了解福建口岸汉坦病毒的基因型别和分子特征。方法对2010年福建口岸捕获的鼠形动物进行汉坦病毒检测,对部分阳性标本进行M基因片段序列分析。结果 2010年福建口岸共捕获鼠形动物839只,以褐家鼠为优势鼠种,占58.52%;鼠形动物携带汉坦病毒阳性率5.84%,均为汉城型(SEO),阳性鼠种为褐家鼠和黄胸鼠,阳性率分别为8.96%和3.09%。汉坦病毒检出呈较明显的冬春季高峰。用M基因片段(nt2001～2301)核苷酸序列构建系统进化树,将福建口岸SEOV分为S2和S3两个亚型。结论 S3亚型SEOV为福建口岸优势流行亚型。
Objective To identify the genotype and analyze molecule characteristics of main Hantavirus in Fujian port. Methods Nested RT-PCR was used to detect Hantavirus-specific nucleic acid sequences in rodents captured in Fujian port, and M segment was sequenced and analyzed in positive samples. Results A total of 839 rodents were trapped in Fujian port in 2010. Rattus norvegicus was the dominant species accounting for 58.52%. The positive rate of Hantavirus in captured rodents was 5.84%, which were all of Seoul Virus （SEOV）. Hantavirus positive species were mainly R. norvegicus （8.96%） and R. tanezumi（3.09%）. Generally, Hantavirus infection significantly increased during winter and spring. The phylogenetic analysis based on partial M segment （nt2001-2301） sequence in 25 samples indicated that there were two genetic subtypes, Seoul 2 and Seoul 3. Conclusion Seoul 3 is the main hantavirus subtype carried by rodents in Fujian port.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
2012年第4期292-294,共3页
Chinese Journal of Vector Biology and Control
基金
国家质检总局科研基金项目(2010IK217)
福建检验检疫局科研基金项目(FK2010-15)~~
