摘要
目的研究肝细胞核因子-κB(NF-κB)在非酒精性脂肪性肝炎中的作用,并探讨丹参注射液对大鼠非酒精性脂肪性肝炎的保护机制。方法采用高脂饮食16周建立大鼠非酒精性脂肪性肝炎模型,并用丹参注射液5ml/(kg.d)进行干预治疗,观察其对非酒精性脂肪性肝炎大鼠肝细胞核因子-κB表达及病理变化的影响。结果丹参治疗组血清ALT、AST分别为(87.6±13.4)、(160.7±32.5)U/L,较模型组的(102.1±31.1)、(210.3±30.2)U/L明显降低,差异均有统计学意义(P<0.05)。模型组肝组织出现重度脂肪变性,不同程度的炎细胞浸润及坏死,丹参治疗组肝组织炎症程度较模型组明显减轻,丹参组炎症计分(4.85±0.39),模型组炎症记分(6.30±0.51)(P<0.05)。丹参治疗组NF-κB表达为(1.77±1.07),较模型组的(5.63±1.45)显著降低,差异有统计学意义(P<0.05)。结论肝细胞核因子-κB表达增强与非酒精性脂肪性肝炎的发生密切相关,丹参注射液能抑制肝细胞核因子-κB表达而达到治疗非酒精性脂肪性肝炎的目的。
Objective To investigate the effect of Salvia on the expression of nuclear factor-κB （NF-κB） in rats liver with non-alcoholic steato-hepatitis. Methods Non-alcoholic steato-hepatitis SD rat model was established by administering a high fat diet for 16 weeks and the rats were injected with Salvia at a dose of 5 ml/kg body weight /d. Pathological changes of liver tissues were determined by HE staining and the expressions of NF-κB in the liver was detected with immunohistochemical method. Results At the end of the 16th week,the levels of serum ALT and AST were significantly lower （P〈0.05） in the Salvia treatment group than the model group. The level of serum ALT in the model group and the Salvia treatment group was （102.1±31.1） and （87.6±13.4）U/L, respectively. The level of serum AST in the model group and the Salvia treatment group was （210.3±30.2） and （160.7±32.5）U/L, respectively. Severe steatosis inflammatory cell infiltration and necrotic loci were observed in the model group. The degree of fatty liver and inflammation were dramatically reduced （P〈0.05）. The inflammatory score was （4.85±0.39） in the Salvia treatment group and 6.30±0.51 in the model group. The expression level of NF-κB was significantly lower （P〈0.05） in the Salvia treatment group （1.77±1.07） than in the model group （5.63±1.45）. Conclusion Salvia may prevent non-alcoholic steato-hepatitis through the reduction of the expression of NF-κB.
出处
《热带医学杂志》
CAS
2012年第8期940-942,964,F0002,共5页
Journal of Tropical Medicine
基金
深圳市龙岗区科技局科研基金(YLL2010034)
