摘要
对采用凯氏定氮法测定灵芝(Ganoderma lucidum)破壁孢子粉中粗蛋白含量的不确定度进行评定,结果表明合成相对标准不确定度为0.02%,扩展不确定度为0.04%。在本次孢子粉粗蛋白质测定中,引入不确定度的主要因素有盐酸标准溶液的浓度及样品测定中实际消耗的标准溶液体积。
We evaluated the level of uncertainty associated with the measurement of crude protein in Ganoderma lucidum wall-broken spores using the Kjeldahl method.Our data revealed that the combined related standard uncertainty and the expanded uncertainty values were 0.02% and 0.04%,respectively.The level of uncertainty associated with the measurement of crude protein in G.lucidum wall-broken spores is mainly determined by the concentration of the standard hydrochloric acid solution and the volume of acid consumed.
出处
《食用菌学报》
北大核心
2012年第2期73-76,共4页
Acta Edulis Fungi
关键词
凯氏定氮法
灵芝破壁孢子粉
粗蛋白质含量
不确定度评定
Kjeldahl method
Ganoderma lucidum wall-broken spores
crude protein content
uncertainty evaluation