摘要
目的 研究人血清中糖基化终末产物 (AGE)水平与肾功能异常的关系。方法 用晶状体蛋白与葡萄糖保温合成晶状体蛋白AGE并免疫家兔获得抗血清 ,通过竞争性ELISA测定正常对照组与晚期肾炎组血清中AGE。结果 正常对照组血清AGE水平为 (2 5 0± 15 ) μg/L ,晚期肾炎组血清中AGE浓度明显升高为 (10 0 0± 38)μg/L ,是正常对照组的 4倍。
Objective To study the relationship between the serum levels of advanced glycation end products (AGE) and abnormal renal function.Methods Anti-serum was gained by immunizing rabbits with AGE-crystallin. The serum levels of AGE in normal and end-stage nephritis were measured by competitive ELISA.Results The serum level of AGE from the patients with end-stage nephritis was significantly increased[(1?000±38) μg/L],and was 4 times higher than that from normal individuals[(250±15) μg/L ,P<0.05].Conclusion The accumulation of AGE may be related to the renal function impairment.
出处
《徐州医学院学报》
CAS
2000年第2期94-96,共3页
Acta Academiae Medicinae Xuzhou
基金
江苏省教委留学回国人员启动基金
关键词
糖基化终末产物
晚期肾炎
ELISA
advanced glycation end products(AGE)
competitive enzyme-linked immunosorbant assay
end-stage nephritis
