摘要
收集20份蓖麻栽培品种,以湖南永州野生种为对照,利用RAPD标记和GC MS法分析亲缘关系,并测定蓖麻籽脂肪酸组分。结果显示:①21条有效引物RAPD PCR扩增获得的总谱带数为75条,多态性谱带数为45条,多态性谱带比例为54.27%,利用UPGMA类平均法对扩增出的谱带进行遗传聚类分析,当遗传距离为0.146 8时,21份材料能较好地聚类;②蓖麻籽油主要含蓖麻油酸、油酸、亚油酸和少量的棕榈酸、硬脂酸、亚麻酸;在蓖麻籽脂肪酸中,蓖麻油酸含量为72.82%～89.54%、油酸2.97%～8.54%,亚油酸3.47%～10.55%,棕榈酸、硬脂酸含量约为1%,亚麻酸含量约为0.5%,品种间蓖麻油酸的含量差别较大。
Phylogenetic relationship and fatty acid components of 21 cultivars （hybrids） and 1 wild Yongzhou variety of Ricinus communis were investigated. The results showed that： ①twenty RAPD primers were picked out from 296 10-mer primers for RAPD analysis; and 73 bands were obtained by use of the 20 primers, among the bands, 42 had polymorphism, accounting for 57.7%; the result of cluster analysis by use of UPGMA method showed that 21 genotypes could be classified together with genetic distant value of 0.1468. ②The castor oil contained mainly ricinoleic acid, oleic acid and linoleic acid, the contents of which were 72,82% - 89.54%, 2.97% - 8.54%, 3.47% - 10.55%, respectively; there was little palmitinic acid, stearinic acid and linolenic acid in castor oil; the respective content of the palmitinie acid and stearinic acid was about 1%, and the content of the linolenic acid was around 0.5%. The content of ricinoleic acid among different cultivars ofRicinus communis showed big differences.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2012年第4期373-376,共4页
Journal of Hunan Agricultural University(Natural Sciences)
基金
国家"973"计划项目(2011CB111514)
关键词
蓖麻
栽培品种
亲缘关系
脂肪酸组分
Ricinus communis
commercialized castor varieties （hybrids）
phylogenetic relationship
fatty acid components
