摘要
目的建立高效液相色谱梯度洗脱法同时测定复方间苯二酚乳膏中间苯二酚和醋酸曲安奈德含量的方法。方法使用ZORBAX Eclipse XDB-C18(4.6 mm×250 mm,5μm),流动相为乙腈(A)-0.5%醋酸铵溶液(B),进行梯度洗脱,0～3 min(25%A),3～5 min(25%A～55%A),5～15 min(55%A),柱温40℃,流速1.0 mL·min-1,检测波长240 nm。结果间苯二酚和醋酸曲安奈德的线性范围分别为:103.18～515.90μg.mL-1(r=1.000 0),1.336～6.680μg.mL-1(r=0.999 4);平均回收率分别为99.3%,99.1%;RSD分别为0.62%,0.73%。结论该方法简便,快速,准确,用于实际样品的测定,结果满意。
OBJECTIVE To establish an HPLC method with gradient elution for the determination of resorcinol and triamcin- olone acetonide acetate in compound resorcinol cream. METHODS The HPLC was conducted on a ZORBAX Eclipse XDB-C18 column （4.6 mm× 250 mm, 5 μm） with acetonitrile （A）- 0.5% ammonium acetate solution （B） as the mobile phase. The gradient elution program was as follows： 0 -3 min （25% A） , 3 -5 min （25% A -55% A） , and 5 - 15 min （55% A）. The column temperature was 40 ℃. The flow rate was 1.0 mL · min^-1 and the detection wavelength was 240 nm. RESULTS The linear ranges of resorcinol and triamcinolone acetonide acetate were 103.18 - 515.90 μg · mL^-1 （ r = 1. 000 0 ） and 1. 336 - 6. 680 μg· mL^-1（r=0.999 4）, respectively. The average recoveries were 99.3% （RSD=0.62%） and 99. 1% （RSD= 0.73% ） , respectively. CONCLUSION The proposed method is simple, rapid and accurate. It has been applied in the assay of samples with satisfactory results.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2012年第15期1254-1256,共3页
Chinese Pharmaceutical Journal
