摘要
运用Real-time Q-PCR方法分析了Frizzed1-10mRNA在小鼠动情周期卵巢中的相对表达水平.结果发现10个Frizzed家族基因中,除Frizzed8外,其他均有不同水平的表达.其中Frizzed1和2在动情前期表达水平显著高于其他3个时期水平,约是间情期水平的4倍(P<0.05);动情前期和间情期的Frizzed3表达水平约是另2个时期的1.5倍(P<0.05);Frizzed4在动情期表达水平显著高于其他3个时期,约是间情期的3倍(P<0.05);间情期Frizzed5表达水平显著高于动情前期和动情期,约是动情期的5倍(P<0.05);Frizzed7表达类似于Frizzed5,间情期表达水平约是动情前期的4倍(P<0.05);Frizzed10的表达在动情前期表达水平约是动情期的3倍(P<0.05);Frizzed6和9在4个时期的表达无显著差别(P<0.05).以上结果表明:Frizzeds在4个动情时期卵巢中广泛而差异表达,并推测Frizzed1—4可能在动情周期卵泡发育过程中具有重要作用.
Real-time Q-PCR was used to analyze the relative expression levels of Frizzed 1-10 mRNAs in the mice ovaries at different estrous stages.The results showed that they had different expression patterns except Frizzed 8.Expressions of Frizzed 1 and 2 were the highest in proestrus and both were about 4 times higher than those in diestrus（P〈0.05）.Frizzed 3 was 1.5 fold higher in proestrus and diestrus than those in the other two periods（P〈0.05）.Frizzed 4 was found significantly higher expression in estrus,which is about 3 fold than that in diestrus（P〈0.05）.Frizzed 5 was marked higher in diestrus than those in proestrus and estrus,being approximately 5 times higher than that in estrus（P〈0.05）.Frizzed 7 was similar to Frizzed 5,about 4 fold higher than the level in proestrus（P〈0.05）.Frizzed 10 expression in proestrus was about more than 3 times higher than that in estrus（P〈0.05）,but Frizzed 6 and 9 were no significant difference during the estrous cycle（P〉0.05）.These results suggested that Frizzeds were widely and differentially expressed in estrous ovary and Frizzed 1—4 may play a more important role in the development of follicle during the estrous cycle.
出处
《福建师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2012年第4期76-81,共6页
Journal of Fujian Normal University：Natural Science Edition
基金
国家863高技术研究发展计划资助项目(2006AA10Z136)
国家自然科学基金资助项目(31101032)
教育部博士点基金资助项目(20113503120002)
福建省自然科学基金资助项目(2011J01144)
福建省教育厅杰出青年基金资助项目(AJ11041)
芜湖职业技术学院引进人才科研基金资助项目
